656 Miyake . — The Fertilization of 
a melting-point of 54 0 , in which it was finally imbedded. 
Sections, from 2 to 5 \x in thickness, were cut with a Minot- 
Zimmermann revolving microtome, and fastened to the slide 
with albumen fixatives. 
Among several staining combinations used, Flemming’s 
triple stain gave the best results, and it was very extensively 
used throughout the research. Heidenhain’s iron-alum 
Haematoxylin was sometimes used, and it gave some good 
results. Hartog’s (’ 95 ) nigrosin-carmine stain, as used by 
Wager (’ 96 ) and Berlese (’ 97 ), was tried on material fixed in 
sublimate solutions, but the results were not very satisfactory. 
Development of the Sexual Organs. 
Nuclei are found irregularly distributed in the protoplasm 
in all parts of the mycelium. The shape of the nucleus is 
spherical or more or less oval (Plate XXXVI, Fig. 1). But in 
some parts of the mycelium the nuclei become considerably 
elongated and deformed. They contain the granules of chro- 
matin, and no nucleoli seem to be present, so far as I am 
able to determine by the staining reactions. No haustoria are 
found in any part of the mycelium. 
The oogonia are formed as terminal or intercalary swellings 
of the hyphae (Figs. 2, 3). Large quantities of protoplasm 
and numerous nuclei pass into them from the hyphae, and 
when they have reached their full size they are cut off from 
the hyphae by a wall, or, if their position is intercalary, by two 
transverse walls (Figs. 4, 5«, 6). 
About the same time that the oogonia are cut off one 
or more antheridia begin to develop close to each oogonium. 
The antheridia are of two kinds, stalk-antheridia and branch- 
antheridia. The stalk-antheridium, the simpler of the two, is 
formed from the part of the hypha next to the oogonium, by 
cutting off an elongated cell, one end of which is thus in con- 
tact with the wall of the oogonium, and its contents are only 
separated from those of the oogonium by the wall of the 
latter, as in Monoblepharis . The terminal oogonium is there- 
fore placed upon it as on a stalk-cell, which may eith:r 
