a parasitic Fungus on Sugar-Cane and Cacao . 689 
them in less than two or three weeks. In one case, however, 
a pycnidium was examined from a ten-days-old culture on 
oak-wood, and stages were observed in spore-formation. The 
spores are liberated after vacuoles have appeared in the 
conidiophores. A portion of the hymenium of this artificially 
grown pycnidium showing spores and paraphyses, is also 
represented in Fig. 9. 
No further developments were noted in the various cultures, 
which were kept under observation for some months. 
It now became necessary to carry out infection-experiments 
with pure cultivations of the Fungus, and to determine to what 
extent it can behave as a parasite. Fully developed and ex- 
tremely vigorous seedling-canes of the variety known as ‘ Bar- 
badoes 347 * or ‘ B. 347 9 were selected for the experiment. 
Incisions were made with a sterile knife in the centre of one of 
the internodes, about halfway up the stem of the six canes se- 
lected for the experiment, after cleaning the internode carefully 
with alcohol. A small chamber was cut under the rind, into 
which portions of mycelium from a seven-days-old pure culture 
of the Fungus in the cane-extract medium were introduced, 
together with some of the food-material. The wounds were 
then bound up with budding-tape. Four canes were infected 
with mycelium, while the other two were treated similarly 
except that no mycelium was introduced and thus served as 
control canes. The result was most marked. In four days 
all four inoculations of the infected canes had taken, and the 
mycelium could be traced in the parenchyma of the internode 
for about two inches in all directions round the point of infec- 
tion. In two cases pycnidia had formed under the rind near 
the point of infection. The control experiments gave negative 
results, and no reddening of the tissues was observed in the 
four infected canes. 
This result entirely confirmed previous infection-experi- 
ments made on sugar-canes in Barbadoes in the early part of 
1900, when the Fungus was found to behave parasitically. 
