692 Howard. — On Diplodia cacaoicola , P. Henn. ; 
ance. The appearance of the mycelium, when eight days old, 
closely resembles that shown in Fig. 8. No further develop- 
ment was noted in hanging-drops, except that the thick-walled 
oil-containing hyphae became dark brown three weeks after 
sowing, and appeared identical with the old mycelium de- 
scribed above in the case of the cane Fungus. As in the 
sugar-cane Fungus, advantage was taken of the aerial develop- 
ment of mycelium in single-spore hanging-drops to prepare 
cultures free from Bacteria. 
In plate-cultures in the cane-extract medium a copious^ 
development of greyish mycelium was obtained on the third 
day, which formed a dense velvety pile, a quarter of an inch 
in height, on the surface of the gelatine, and in which dark 
bodies could be detected with the naked eye. When examined 
on the sixth day these dark bodies were found to be pycnidia 
in which paraphyses and spore-formation could be detected, 
after the manner indicated in Fig. 9. 
On sterilized cacao- and oak-wood small dark bodies were 
noted in nine days, and these when twenty days old proved 
to be the pycnidia of the Fungus. 
It will be seen therefore that the development, under arti- 
ficial conditions, of the Fungus of the cacao tree corresponds 
exactly in all its details with that found on the sugar-cane, 
so that morphologically regarded the two forms are identical. 
It was now necessary to perform infection-experiments on 
cacao trees and pods, with pure cultivations of the Fungus. 
These were as follows : — 
3. On cacao pods : 
(a) Two nearly ripe pods were selected for the experiment, 
and were washed with alcoholic corrosive sublimate, and at 
the points where incisions were to be made small cavities were 
made in the rind by lifting the surface and cutting out a small 
portion of the tissues underneath. Into one of these chambers 
actively growing mycelium, three days old and from a pure 
culture, was introduced and the pod was bound up with 
budding-tape. The other pod was treated in a similar way, 
except that no mycelium was introduced and thus served as 
