a parasitic Fungus on Sugar-Cane and Cacao. 695 
tight bandage of budding-tape. The control plants were 
treated in the same way, except that no mycelium or spores 
were purposely introduced into the cavity. 
(a) Two branches of a healthy cacao tree were selected 
for the preliminary experiment. In one cavity actively 
growing mycelium in the cane-extract medium was introduced, 
while the other branch was used as a control. Eight days 
after infection the Fungus had killed the bast up to about 
eight inches above and below the chamber, at which point 
the branch was nearly ringed. The mycelium could be 
traced more than a foot above and below the chamber, both 
in the bast and in the wood, and to a considerable depth in 
the latter. Numerous pycnidia were developed under the 
attacked bark, some of which were liberating their spores. 
The control showed no infection. 
(b) Eight healthy cacao plants about eight months old, 
growing in bamboo pots, were next selected for an experi- 
ment. Nos. 1 and 2 were infected with spores taken from 
a pod attacked by the Fungus, 3 and 4 with portions of the 
diseased rind of a pod containing actively growing mycelium, 
4 and 5 with vigorous mycelium from a pure culture in cane- 
extract and 7 and 8 were control plants. After binding up 
the wounds the plants were placed in the shade and watered 
daily. Eight days afterwards it was found that infection had 
taken place in all the plants from 1 to 6, while the control 
plants showed no infection. There was scarcely any dif- 
ference in the amount of infection when spores or diseased 
cacao-pod rind were used, except that plant No. 1 was 
killed outright by the Fungus. When culture-mycelium was 
used infection was more extensive, one of the plants being 
killed while the other was evidently dying. In each case 
(5 and 6) pycnidia were formed under the rind. 
(c) Four vigorous young trees about eighteen months old 
were now selected. The first was used as a control, the 
second was infected with spores, the third with a portion 
of a diseased pod taken from near the still healthy tissue, 
and the fourth with pure culture- mycelium. Eight days 
