Beer. — Studies in Spore Development. II. 715 
the interior of the chromosome bodies and surrounds these with the first 
karyolymph. Where this karyolymph meets the cytoplasm a precipitation 
membrane is deposited, and this constitutes the first nuclear wall. Sub- 
sequent to this, materials not only pass through the membrane from the 
nucleus to reach the cytoplasm, but the karyolymph is without doubt 
reinforced by materials which reach it from without. 
As the chromosomes of Tragopogon and Crepis, which were at first 
crowded so closely together, begin to separate, their viscous substance 
remains attached at certain spots, and at these points of union the material 
of the chromosomes becomes drawn out into connecting bars and arms 
which become longer and finer as the chromosomes move further apart. At 
the same time that this is taking place vacuoles appear within the bodies of 
the chromosomes. These vacuoles not infrequently occupy the central 
region of the chromosome, and their gradual growth and union tends, 
in these cases, to divide the chromosome into two longitudinal halves 
(Fig. 34). This corresponds to the behaviour of the chromosomes observed 
by Miss Digby ( 4 ) during the telophase of the heterotype division in 
Galtonia. 
In other cases, however, such a regular arrangement of the vacuoles 
along the middle of the chromosomes is not apparent, and these bodies re- 
solve themselves into an irregular reticulum without any distinct separation 
into two longitudinal halves (Fig. 35). As the telophase proceeds the 
material of the chromosomes gradually becomes more and more distributed 
along the anastomosing bars and filaments, and at the same time opens out 
by the increase in alveolization. Complete 4 rest * does not appear to be 
reached in any case, however, for distinct traces of the chromosome bodies 
of the preceding division can be seen right up to the time that the prophases 
of the homotype division are entered upon. Figs. 36 and 37 represent two 
daughter nuclei of Tragopogon , as near the 4 resting ’ stage as they ever 
come. 
In Tragopogon and Crepis , however, the resolution of the chromosomes 
goes comparatively far, but in Matricaria the chromosomes maintain almost 
their entire independence during interkinesis. In this case we might 
justifiably speak of the existence of prochromosomes in the nucleus. 
On first reaching the poles of the spindle the chromosomes of Matri- 
caria are closely crowded together, but a little later they again separate 
somewhat from one another. During this separation portions of their 
viscous substance remain adherent and become drawn out as the chromo- 
somes move apart, so that these bodies are connected together during 
interkinesis by filaments of chromatic material. The formation of these 
connecting branches often gives the chromosomes an irregular, almost 
amoeboid, appearance during interkinesis, but they show no indication 
of alveolization or of becoming otherwise broken up (Fig. 33 b). The 
