728 
Lee. — Observations on the 
seedling anatomist, showed that the collet could be defined anatomically, 
and referred very briefly to the vascular anatomy of a few species among 
the Sympetalae (3) ; and about twenty years later Van Tieghem (25, 26) 
described the transition phenomena in a few examples from the same 
group. A decade later was published an account of the careful work of 
Gerard (7), which included a description of the seedling structure of nearly 
a score of examples belonging to the Sympetalae. Several of the same 
species have been included in the present research, which, except in details, 
confirms and extends this branch of Gerard’s work. Of Vuillemin’s memoir 
on the Compositae (28) more will be said in a future paper on that group. 
P. A. Dangeard (5), who extended Gerard’s observations on the Sympetalae, 
classified his results on the basis of the number of strands present in the 
root ; while Van Tieghem, in defining his three types of transition (27), also 
included a few examples from the same group. In 1890 Scott (18) described 
the seedling anatomy of Ipomoea versicolor ; and a little later Scott and 
Brebner, in their paper ‘On Internal Phloem in . . . Dicotyledons’ (19), and 
Lamounette (12), working independently on the same subject, incidentally 
included a description of the transition phenomena in various Sympetalae. 
It remains only to mention the more recent publications of Tansley and 
Thomas (21, 22), and Thomas (23), in which the seedling anatomy of cer- 
tain members of this group is described in a more general manner. 
Scope and Methods. 
In the present paper only a few of the better-known orders of the 
Tubiflorae will be considered; in these the seedling anatomy of selected 
species will be described, and the results of other workers will be included. 
The methods adopted are similar to those described by Hill and de 
Fraine (10). When possible, series of transverse sections of three seedlings 
of each species were cut with the microtome, and longitudinal sections were 
also made for comparison. For staining, combinations of gentian violet 
and vesuvin, and safranin and lichtgrtin, were found to give the best 
results. 
For the majority of the seedlings and for much kindly advice as to 
methods I am indebted to Mr. T. G. Hill. The greater portion of the 
material was grown at the Chelsea Physic Garden, and to Mr. W. Hales, 
the Curator, I wish to express my sincere thanks. 
Description of Species. 
Convolvulaceae. 
Convolvulus tricolor , L., var. major. Seedlings large, each with massive 
hypocotyl (often more than 6 cm. long) and two slightly unequal cotyledons 
(PI. LXVIII, Fig. 1, a). Each cotyledon consists of a long slender petiole 
