890 
Miller . — A Physiological Study of the 
those investigations which are deemed necessary for a clear discussion of 
the results obtained. 
Stages of the Seedling examined. 
The oil was examined in the resting seed and in five different stages of 
the seedling. The stages examined were the same as those studied in the 
previous investigation with the exception of Stage I. Previously the first 
examination of the seedling was made when the hypocotyls and roots had 
attained a length of 2*5 to 3-5 cm. Experience showed, however, that it 
was difficult to obtain a sufficient quantity of hypocotyls and roots to yield 
the amount of oil necessary for the determinations that were to be made. 
The seedlings, therefore, were first examined at a later stage of development, 
when the roots and hypocotyls had a length of 3-5 to 5 cm. The seedling 
reached this stage in about three and a half to four days. 
A brief description of the last four stages of the seedling examined is 
given here for convenience. If a more detailed explanation is desired, the 
description and figures of the different stages may be consulted in the 
former paper. 
Stage II. The hypocotyls and roots had a length of from 7-5 to 1 1 cm., 
and the arch of the hypocotyl was just breaking through the surface of the 
ground. The cotyledons had not yet emerged from the seed-coat. The 
seedlings reached this stage about five days after the planting of 
the seed. 
Stage III. At this stage the hypocotyls had reached a length of from 
5 to 6 cm. above the surface of the ground, and the main roots were about 
the same length with numerous side roots about 2-5 to 3*5 cm. in length. 
The cotyledons were a bright green, and were spread out perpendicular to 
the hypocotyl. The plumule had not yet developed. The time required 
for the seedling to reach this stage was about seven days. 
Stage IV. The hypocotyls had now a length of from 8 to 10 cm. from 
the surface of the ground to the cotyledons. The main roots had reached 
a length of 6 to 7 cm., and had developed an abundant growth of lateral 
roots. The cotyledons had broadened and lengthened, and the plumule 
was slightly developed. The young plant reached this stage of development 
in about ten days after the planting of the seed. 
Stage V. The seedling at this stage differed little from that of Stage IV 
in general appearance, except that the plumule was more fully developed, 
and the cotyledons had become more leaf-like. The seedling reached this 
stage in about fourteen days after the seed was planted. 
Preparation of Material for Analysis. 
I. Preparation of Dry Material. The seedlings for this experiment 
were grown in ordinary quartz sand in the greenhouse. As soon as the 
