Brenchley . — The Development of the Grain of Barley . 927 
reached at about the time at which desiccation sets in. Then, while the nitrogen 
and phosphoric acid remain fairly constant, the ash decreases somewhat in 
quantity. The phosphoric-acid-starved plot gives somewhat abnormal 
results, differing in some respects from those of the other plots. 
3. The long growing period of barley gives a prolonged period of desic- 
cation, during which certain maturation changes are evident. These are 
hardly seen in wheat, as the latter crop is cut just when they are beginning. 
4. With wheat the manuring had very little effect on the analyses of 
the grain or straw, whereas with barley the effect of phosphoric acid starvation 
is reflected in the results obtained. 
5. The infiltration of starch follows a progressive course from the 
chalazal end of the grain up towards the embryo, the cells in the flanks of 
the grain being the first to show signs of the carbohydrate. 
6 . As the barley grain develops nuclear changes set in, due probably 
to the pressure of the increasing bulk of starch grains. The nuclei first lose 
their nucleoli and then gradually get deformed and squeezed out into net- 
works of varying degrees of coarseness. The deformation seems to progress 
from both ends of the grain simultaneously towards the middle, the last cells 
to be involved being those of the sub-aleuronic layer of the endosperm. 
