Essed . — The Panama Disease. /. 
347 
spicuous, unbranched conidiophore as in Gibberella cyanogena . At the 
same time thin curling branches appeared, at the apices of which arose 
round cup-shaped chlamydospores with granular, richly vacuolated proto- 
plasm. On the tenth day some thick hyphae appeared, giving*rise to the 
same chlamydospores, intercalary as in Chlamydomucor . In one case they 
were wide apart, in another in immediate contact with each other, so that 
fusion took place, producing multicellular chlamydospores as alluded to 
before. On another culture they were seen to arise on the apex of short 
thick branches, as in Hypomyces ochraceus (see Fig. 7). 
Anticipating this kind of chlamydospore formation, the protoplasm of 
the hyphae was drawn to favoured sites, where the spores would arise, and 
the interspaces were left entirely empty and finally collapsed, liberating the 
chlamydospores. Their colour when mature is dark-brown or violet ; the 
exosporium is generally smooth, but now and then adorned with numerous 
warts. In some instances the hyphae did not produce resting spores, but 
broke up into their component cells, giving rise to oidia-like reproductive 
organs. 
The sclerotia arose on extremely thin hyphae as transparent yellowish 
saucer-shaped bodies, measuring +ioojut across. The cultures were then 
nearly five weeks old. Sometimes they stood solitary, then again they 
were crowded together, and finally fused into a massive gelatinous body, in 
which no separating membranes, but often the above-mentioned globular 
bodies, were visible. Maturing, they assumed a darker colour and hardened 
into the identical gristly structures met with in the histological preparations. 
In the same way they broke up into identical chlamydospores. In the 
absence of fusion some of the single scyphoid sclerotia matured into large, 
dark-coloured, spore-like bodies, as mentioned before on p. 34 6 ; I shall term 
them giant chlamydospores. They may be expected to have the poten- 
tialities of a single scypho-sclerotium or of a group of chlamydospores that 
could arise out of such a sclerotium. The other mode of sclerotium forma- 
tion hinted at in the description of the sections is readily explained by 
Figs. 1 and 6. 
The process of chlamydospore-formation is initiated by pellucid rings 
within the sclerotia. The mass then splits in polygonal plates, each con- 
taining one or more spores ; at last they are completed, still surrounded by 
a thin layer of mucilage, which is finally resorbed. Indeed, the whole pro- 
cess is identical with that of the Ustilagineae. 
The second kind of mycelium was marked off from the first one by the 
dusky colour of the central underlying part and the conspicuous hemi- 
spherical shape. The dark colour was due to the basal growth, consisting 
of thick hyphae of a smoky hue. They terminated, however, in hyaline 
slender apical portions, and the secondary mycelium arising on them was 
also perfectly hyaline. On the seventh day chlamydospores arose, cut off 
