352 
Essed . — The Panama Disease . /. 
Bouillon culture . Yellowish-green fluorescence; sediment in condensa- 
tion liquid abundant, slimy ; can only be divided by agitating well. Skin 
on the surface, yellow-green colour. 
Milk culture. Clotting after nine days ; acid reaction. 
Potato culture. Flat, dull grey ; margins sinuous. No gas and no indol 
formed. 
Microscopic appearance. In hanging drop, short but slender rods, 
sometimes long threads and stout forms, all of which displayed great 
motility. 
Staining. Gram-negative. One terminal cilium found by using 
Bunge’s mordant and staining with fuchsin. 
It is plain that this micro-organism is nothing but a form of Bacterium 
fluorescens. Reasoning from facts gathered during my research, it looks to 
me at least improbable that Bacteria have any share in the ruinous war 
waged on the banana plantations in Surinam. 
To satisfy all conditions, however, I inoculated four suckers with the 
fungus-spores, four with Bacteria, and four with fungus-spores and Bacteria 
combined. Which will prove to be the mischief-maker time will tell. 
EXPLANATION OF FIGURES IN PLATE XXVIII. 
Illustrating Mr. Ed. Essed’s paper on the Panama Disease. Part I. 
Fig. i. ( [w .) wood vessels full of hyphae spores, and sclerotium forming; ( s .) sieve tubes 
plugged by sclerotium, spores forming; (sc.) scyphoid sclerotium. 
Fig. 2. Haustoria in parenchyma. X 105. 
Fig. 3. (a) spore-clusters; (/>) sclerotium in intercellular space broken up into chlamydospores ; 
(c) giant chlamydospores. 
Fig. 4. Stellate cell in which sclerotia and spores are formed. 
Fig. 5. Section through leaf, showing sclerotia and spores. 
Fig. 6. Explained in text. 
Fig. 7. Explained in text. 
Fig. 8. Explained in text, x 255. 
Fig. 9. (1) Fruit body on sclerotium. x 10. (2) Young stroma with spores and paraphyses, 
(3) Nearly mature stroma. 
