368 Essed. — Rice Disease caused by U stilaginoidell a graminicola. 
Fig. 2). The prosenchyma and the wood vessels are only seized upon in 
a far advanced stage of the disease, when pegmatia and small chlamydo- 
spores of irregular shape and greenish brown colour may be seen to form 
within the vessels and sclereids. 
Pure cultures were not easily obtained, on account of the overwhelming 
number of bacterial colonies overrunning the mycelia. But when the 
leaves were first rinsed under the tap for a long time, steeped in 90 per cent, 
alcohol for two minutes, and washed out again under the tap for some time 
so as to remove the alcohol, and at last dried in the stove at a temperature 
of 40° C. for two days, the leaf fragments could be easily pounded into 
a coarse powder, which could be shaken with a sufficient quantity of steri- 
lized water so as to obtain a suitable inoculation fluid. A few drops of the 
liquid, strained through a linen cloth previously steeped in boiling water, 
were poured on four Drigalsky plates and spread. The results were 
gratifying ; two days after inoculation I was able to transfer nine pure 
growing mycelia. 
Since the differences between this and the two foregoing Fungi are not 
very great, it will suffice to cite these differences, so avoiding unnecessary 
prolixity. 
Pegmatia. Not differing ; the chlamydosporangioid form smaller. 
Spores. Chlamydospores smaller than in the two other species ; colour 
greenish brown. Chlamydosporangia smaller, but of the same hue as in 
the two other species. Conidia 1-5 celled, sickle shaped, rather larger than 
in the two other species. As typical for this fungus may be considered the 
1-, 3-, and 5-celled conidia, the 2- and 4-celled ones being scarce. Conidio- 
phores as in Ust. oedipigera . 
Haustoria , as in the last-named fungus. 
Enzyme. The enzyme obtained in the ordinary way was only capable 
of gelatinizing the cell-walls ; no proteolytic action was discovered. 
Hyphal walls, as in Ust. oedipigera. 
The results of the inoculation experiments will be mentioned later on. 
EXPLANATION OF FIGURES IN PLATE XXXI. 
Illustrating Mr. Ed. Essed’s paper on Rice Disease. 
Fig. 1. Diseased spots in leaf, x 6. 
Fig. 2. Transverse section of leaf. ( A ) pegmatia in parenchyma; ( B ) spores in emergences and 
stoma, x 255. 
Fig. 3. (a) Fertile hyphae with conidia, chlamydospores, and pegmatia ; ( b ) chain of oidia and 
chlamydospores. 
