Wilson, — Spermatogenesis in the Bryophyta . 431 
attached to the outer shell-like portion, and still persists as a deeply staining 
appendage of the limosphere. At or near the distal end of this appendage 
the blepharoplast is found as a small spherical deeply staining body (Figs. 28 
and 29). 
At this point, for the sake of clearness, a recapitulation of the various 
bodies produced from the nucleolus may be given. The first bodies cut off 
pass into the cytoplasm and form the rod-like bodies. During the elonga- 
tion of the latter the nucleolus enters upon a second period of division, and 
the fate of the bodies produced is somewhat doubtful. A third period 
of division results in the formation of two bodies within the nucleus, one of 
which, in all probability, is identical with the blepharoplast. The origin of 
the latter structure was not definitely determined, but there is little doubt 
that it is the last body produced by constriction from the nucleolus. At 
a later stage, as seen in Fig. 27, one of the bodies produced by this final 
division is found in the cytoplasm, and when the formation of the limosphere 
is almost completed no deeply staining bodies can be seen within the 
nucleus. The blepharoplast is now found at the end of the appendage, 
while a second body is present in the cytoplasm in the vicinity of the limo- 
sphere (Figs. 28, 29, 31, and 32). It appears that the substance of the 
nucleolus is completely used up in the production of these bodies, and that 
both pass out of the nucleus, one functioning as the blepharoplast, while 
the other persists without change in the cytoplasm, and is finally found 
in the vesicle of the spermatozoid. It is possible that this second body 
corresponds to the ‘ Nebenkorper’ described by Ikeno (32) in Marchantia , 
but further investigation is necessary before any statement can be made 
with regard to this matter. This body will in future be referred to as the 
accessory body. In view of the considerable number of similar bodies 
present in the spermatoid an earlier origin of the blepharoplast is not 
absolutely excluded. One of the bodies first produced from the nucleolus 
may persist throughout the changes described above, and finally function as 
the blepharoplast, but this is considered improbable. 
In the following description the portion of the spermatid in the vicinity 
of the blepharoplast will be spoken of as anterior ; the limosphere occupies 
the opposite or posterior part, while the appendage connecting the blepharo- 
plast and the limosphere run approximately in an antero-posterior direction. 
Arens (2) has pointed out that in Poly trichum juniperinum the blepharoplast 
is situated on that part of the periphery of the spermatid which is directed 
towards the apex of the antheridium, but in Mnium no such regularity 
in its position has been discovered. The anterior (previously spoken of as 
the distal) end of the appendage extends to within a short distance of 
the membrane of the cell. The blepharoplast is at first found at the 
same spot, but a little later it changes its position and comes to lie 
on the actual periphery. In some cases the appendage elongates and 
