Bailey. — Puccinia malvacearum and the My coplasm Theory . 181 
the rest being kept as controls. No rust appeared on any of the uninoculated 
leaves during the experiment 
One pustule was used for each inoculation, and these were taken from 
leaves which had been kept for some time previously either submerged in 
water or exposed to a damp atmosphere. 
The mode of germination of the teleutospores was ascertained by 
microscopical examination before the pustules were employed for purposes 
of inoculation. 
Date. 
Plant 1V0. 
Inoculation 
Number. 
Table I. 
Material used for inoculation. 
Nature. Condition. 
Results. 
I 
i 
sporidial 
lion 
germina- 
good 
6 pustules on lower 
surface. 
2 
>5 
99 
77 
1 pustule on lower 
surface. 
3 
j* 
9 ) 
99 
Failed. 
4 
oidial 
99 \ 
oidia showed ten- 
9 9 
5 
99 
\ 
h dency to disor- 
99 
6 
99 
i 
1 ganize rapidly 
99 
2 
7 
sporidial 
>1 
good 
No pustules, but 
several small black 
flecks of dead tissue, 
showing localized 
lethal effects of the 
fungus. 
8 
99 
>7 
77 
99 
Ditto 
9 
99 
99 
Failed — no black 
flecks. 
IO 
oidial 
77 1 
. oidia showed ten- 
Failed. 
1 1 
>> 
1 
77 | 
[ dency lo disor- 
77 
1 2 
99 
J 
’ ganize rapidly 
77 
3 
13 
sporidial 
}> 
good 
3 pustules on lower 
surface. 
14 
99 
» 
99 
i pustules on lower 
surface. 
>5 
99 
99 
99 
] pustule on lower 
surface and con- 
siderable number of 
black flecks. 
16 
oidial 
99 
99 
13 pustules on lower 
surface. 
17 
99 
99 
99 
4 pustules on lower 
surface. 
18 
99 
99 
99 
1 pustule and con- 
siderable number of 
black flecks. 
4 
19 
sporidial 
„ 
9 9 
Failed. 
20 
99 
99 
77 
21 
,, 
99 
99 
77 
22 
oidial 
99 
99 
\ 
, 77 
Failed, but fair 
23 
99 
number of black 
2 4 
7 7 
99 
” i 
flecks. 
5 
25 
sporidial 
99 
99 
1 pustule on lower: 
surface. 
26 
99 
9 9 
99 
2 pustules on lower 
surface and few 
black flecks. 
