Bailey . — Puccinia malvacearum and the My coplasm Theory. 185 
capillarity to ensure germination. On June 7, 1912, one seed was sown 
under each of the globes in the manner described above. 
Six of these seeds failed to germinate or produced sickly seedlings, and 
were replaced by fresh sterilized seed about three weeks later. 
Twelve seeds from the same source, but unsterilized, were sown in small 
pots as controls at the same time as the first sowings were made under the 
globes. Two of these seeds also failed to germinate, but were not replaced. 
The globes were placed in line along a bench in an unheated green- 
house, and were sheltered from direct sun by means of blinds. The control 
pots were placed between the globes along the bench. 
The remainder of the seed was sown in an open bed just outside the 
greenhouse. 
By the end of August two of the plants in the globes had failed — due 
in one case to an accident. 
The remainder of the ‘ globe plants ’ had from four to six leaves apiece, 
and varied from about the same size as the ‘ pot-controls ’ to twice that size. 
The colour of the leaves was scarcely any lighter than that of the 
controls in pots, but the texture of the leaves was obviously thinner. None 
of the plants in globes or pots showed any rust. 
The plants in the outside border, examined three days later, showed 
slightly fuller growth, and practically every plant had a few scattered rust 
pustules on one or more of its leaves. 
One of these slightly diseased plants was potted up on September 3, in 
place of one of the ‘ globe plants * which had failed. The shoot and leaves 
of this plant were pushed through the small neck of the globe, which was 
lowered over it. The fate of this particular plant is interesting, as it shows that 
the conditions inside the globes were highly favourable to the development 
of the fungus. When first placed under the globe it bore three infected 
leaves; the largest of these showed 12 immature pustules, the second 
largest showed one mature pustule, and the smallest showed a couple of 
dozen undeveloped pustules. 
By October 11 the three oldest leaves had died off, presumably killed 
by the fungus: of the remainder, two leaves, each about two inches in 
diameter, were covered with innumerable pustules, whilst three young leaves 
just emerging from the bud were, apparently, free from disease. 
Three weeks later the entire plant was dead and covered with the 
remains of rust pustules. 
This would seem to prove that the conditions inside the globes, though 
certainly abnormal, were not in any way such as would suppress the growth 
of any fungus latent in the tissues of plants growing inside the globes. Two 
features are also well worth noticing in the results just described : (1) that 
a certain development had to be reached by the young leaves before they 
could become visibly infected, and (2) that the density of pustules on the 
