Bailey . — Puccinia malvacearum and the My coplasm Theory. 187 . 
spores taken from one of the ‘ pot-controls ’ growing near at hand. On the 
20th of the same month several pustules of Puccinia were found on the under- 
side of the inoculated leaf, and three other leaves on the same side of the 
plant showed a few pustules. 
The experiment was finally brought to an end on May 22, 1913. On 
this date all the plants were removed from the globes and carefully 
examined. All had formed several new leaves during the spring, and the 
number of Collembolae was much increased. 
None of the plants, however, with the exception of the artificially 
inoculated one just referred to, showed any sign of rust. 
The ‘ pot-controls ’ were also examined on this date. These had kept 
their leaves during the winter and the rust had made steady progress. 
Three of these control plants are -recorded as being ‘ heavily infected ’, two 
as being ‘ moderately infected ’, and the remaining four as £ slightly ’ or ‘ very 
slightly infected ’. 
The plants in the border outside had been grubbed up during the 
winter, and, consequently, no record is available. 
Summary of Results of Experiment 1. 
The cultures were started during June 1912, and were continued until 
the middle of May 1913. No rust appeared on any of the protected plants 
throughout the experiment, though, on Eriksson’s expectation, a ‘ primary ’ 
outbreak might have been expected by the end of September at latest. 
The controls in the same house first showed rust on October 11, but only 
a few pustules. The rust progressed slowly throughout the winter, and by 
April 1913 the majority of these plants showed a fairly heavy infection. 
The controls in the bed outside first showed slight infection on September 3, 
and this increased as the season went on, but never reached ‘ primary ’ 
intensity. 
One of the ‘globe plants’ was inoculated, towards the end of April, 
and gave a positive result. 
At the beginning of September a slightly diseased plant, growing 
amongst the outdoor controls, was potted up underneath a globe. A 
month later some of the leaves of this plant showed extremely heavy 
infection, recalling Eriksson’s description of ‘ primary infection ’. After 
seven weeks the plant had been killed outright. The condition inside the 
globes would therefore appear to be favourable to the fungus. 
Experiment 2. 
In this series of cultures an attempt was made to improve on the con- 
ditions in the former experiments as follows : (1) by installing a system of 
continuous regulated aeration of the globes. (This would also have the 
effect of reducing the humidity of the atmosphere inside the globes.) 
