Bailey. — Puccinia malvacearum and the My coplasm Theory . 197 
APPENDIX. 
Germination Experiments with Spores of 
Puccinia malvacearum. 
Series I. (July 1914.) 
Spores scraped from young pustules on to surface of water in watch- 
glasses. 
Some of the spores sank ; others remained floating on the surface. 
Only such pustules used as were brown in colour or showed only 
a faint trace of grey. 
Pustules taken from six different hollyhock plants, all of which were 
growing in the garden, and had been naturally infected. 
Nine cultures made : 
Spores on surface of water. Spores at bottom^f water. 
Culture. 
Germination. 
Type. 
Germination . 
Type. 
1. Sterile tap-water 
Good. 
Mostly oidial; some 
sporidial. 
Good. 
Entirely oidial. 
2. Distilled water. 
Poor. 
Mostly oidial ; one 
sporidial. 
Poor. 
yy yy 
3* yy yy 
Fair. 
Mostly oidial ; some 
sporidial. 
Moderate. 
yy yy 
4. Sterile tap-water. 
Fair. 
About equal propor- 
tions. One spore showed 
different germination 
from its two cells. 
Fair. 
yy yy 
5 * yy )} 
Moderate. 
Mostly oidial. 
Good. 
Entirely oidial. 
d. ,, )> 
Poor. 
Mostly oidial ; some 
sporidial. 
— 
— 
7' >’ 
Good. 
Mostly sporidial ; some 
oidial. 
Good. 
Entirely oidial. 
8 . )> 
Very poor. 
Apparently all oidial. 
Good. 
yy yy 
9 - » » 
Poor. 
>) 
Poor. 
yy yy 
Series II. (July-August 1914.) 
Entire pustules removed from leaves of naturally infected hollyhock 
plants, and placed on or below the surface of water in watch-glasses. 
Pustules taken from 3 different plants, growing in open bed. 
Culture. 
Spores in pustules on surface. 
Spores in submerged pustules. 
Germination. Type. 
Germination. 
Type. 
1. Sterile tap-water. 
Good. | 
1-4. Mixture of spori- 
dial and oidial ; the 
former preponderat- 
ing. 
Good. 
All oidial. 
2 >> yy 
yy 
yy 
3 yy yy 
yy 
yy 
9) 
4. ,, „ 
yy ' 
yy 
5 * yy yy 
” 1 
5-7. Mixture of spori- 
1 dial and oidial. 
yy 
” 
6. j. 
„ | 
\ In two or three cases 
yy 
jy 
7 * ” yy 
' spores lying just on 
the surface of the water 
showed different types 
of germination from 
the two cells of the 
spore (see Fig. i). 
yy 
yy 
