Saccharomyces anomalies [Hansen). 217 
towards the neck, like a greasy film on the moisture there 
found, the growth appearing somewhat mycelium-like in nature 
when undisturbed. A copious deposit of cells soon forms at 
the bottom of the flasks owing to gradual wetting and pre¬ 
cipitation of the old cells: this deposit is white in colour at 
first, but becomes a dirty-brownish colour in old cultures. 
The liquid itself remains clear, when the film attains a good 
development. 
Culture in tube's. 
Streak-cultures were made on tubes of sloped beer-wort 
gelatine, and grew rapidly. A well-developed streak has in 
most cases a milky-white appearance. It is quite opaque, 
except that part of it which grows on the thin layer of gelatine 
at the upper part of the sloping surface, where the streak is 
semi-transparent and thin, and usually develops a mycelium¬ 
like fringe at its edge. The other portion of the streak is 
thick, moist, and fatty-looking, and its edges are more or less 
crenate and well-rounded. It does not grow down into the 
gelatine, but remains on the surface. In some cases when 
the gelatine dries owing to various causes, the streak has 
a different appearance altogether, being flat, glistening white 
in colour, eventually becoming dry and almost powdery, and 
marked with radial and concentric zones. 
Beer-wort agar 1 has also been used, and the growth on this 
is even more rapid. Streaks similar to the fatty-looking 
streaks on beer-wort gelatine are produced. 
Plate-cultures. 
On beer-wort gelatine. The forms of the colonies are by 
no means constant on different plates: on the same plate,, 
however, as a rule they are fairly constant. This applies not 
only to plates of different series, but to plates of the same 
series, and will be explained below. 
The colonies appear as small whitish dots. Those on the 
1 Agar 2 grams. Wort 90 c.c. 
