220 Barker.—A Fragrant ‘ My coderma ’ Yeast , 
Culture in hanging drops. 
Cells grown under observation in hanging drops of beer- 
wort gelatine were ellipsoid or more or less ovoid in the 
adult stage. They were filled with clear, colourless proto¬ 
plasm, and the extremely thin cell-walls were not distinctly 
marked off from the cell-contents. A single isolated cell may 
easily be overlooked on account of its transparency in the 
gelatine. At a temperature of I9-I9*5°C. a single adult cell 
of the above form produced another similar adult cell in two 
hours by normal budding, the bud arising generally from the 
neighbourhood of the more pointed end. In the case figured 
(Fig. 9) the single cell had produced four similar cells in four 
hours, and in six hours seven cells. The daughter cells are 
at first round, but become usually more ovoid before pro¬ 
ducing another cell. 
As a rule, after a colony of about eight cells was produced, 
the gelatine in the immediate neighbourhood was liquefied, 
with the result that the cells separated from one another, and 
the colony was thus broken up. Owing to the diminution of 
resistance as the gelatine softened, the buds separated from 
the mother cell much earlier than was the case when the 
gelatine remained unliquefied. These young separated buds 
bore a great resemblance to small Torula-like Yeasts. At 
a temperature of 15 0 C. the gelatine was not so rapidly liquefied, 
consequently larger colonies were found before separation, 
some containing a great number of cells. In hanging drops 
at this temperature about thirty-six hours old, the cells at the 
edges of the colonies have a tendency to grow out into a 
false mycelium, becoming longer and more rod-like in appear¬ 
ance. The colony then looks like a many-rayed star (Fig. 5), 
owing to the radiating series of branching cell-series to which 
I have throughout applied the term ‘ mycelium-like.’ 
In hanging drops a week old at 19 0 C. the cells have a 
sharply-marked cell-wall and are more or less vacuolated. 
Many of them contain bright refringent granules, and in some, 
spores were observed, as will be described subsequently. 
