224 Barker—A Fragrant f My coder ma Yeast , 
breadth the pear-shaped forms are 8-5 jot. Many of these 
elongated forms are found, placed end to end, forming a 
false mycelium. These cells with granules are to be found 
in old cultures, both in solutions and on solid media. They 
constitute almost the whole of the vegetation in these cases. 
Among them cells containing spores are usually to be found. 
Illustrations of this type of vegetation are found in Fig. 10, 
a. b. c ., taken from secondary films formed on beer-wort, sac- 
charose-Mayer, and laevulose-Mayer solutions respectively. 
Spores. 
This Yeast is remarkable for the ease with which it can 
form spores and also for the shape of its spores. They are 
produced one to four in number in a single cell, the usual 
number being three or four. They are similar in form to 
those described by Hansen for Saccharomyces anomalus x , 
being shaped like a half-sphere with a horizontally-projecting 
rim round the edge of the flat surface. From the resemblance 
to an ordinary f Bowler ’ or e Billy-cock ’ hat, they may be 
termed hat-shaped. In size they average 3*5 /u,—4-5 M- 
Cells containing spores are always to be found in old cul¬ 
tures, whether on solid media or in liquids. Spores which 
have escaped from their mother-cells are also abundantly 
found: those from the same mother-cell usually remain 
attached by their thickened rims to one another, and show 
the usual tetrad arrangement. 
In order to obtain abundant supplies of spores the following 
method was adopted :—A few drops of a vigorous twenty- 
four hours’ old culture in beer-wort at 28° C. were poured 
over sterilized pieces of porous biscuit-porcelain filter-plate. 
These were allowed to remain at 25 0 C. for forty-eight hours 
in a moist chamber, and on examination of the growth formed 
on the plate numerous spores were found. Abundant spore 
production takes place at any temperature between i8°C. 
and 28° C. Blocks of gypsum were tried instead of the porous 
filter-plate, but the latter gave the better results. 
1 Hansen (i). 
