253 
* 
of Poronia punctata (Z.). 
perithecia, also of Marshall Wards 1 discovery that the some¬ 
what similarly shaped stromata of Onygena produce chlamydo- 
spores on the surface before the asci are developed inside. 
At different levels below the surface of the stroma of 
Poronia are visible circular knots of hyphae, distinguished 
from the surrounding hyphae by their smaller diameter and 
by their staining more readily. Within these knots of hyphae 
are seen much larger and still more deeply stained portions 
of a coiled hypha, which is, in fact, the { Woronin’s hypha ’ 
described by Fuisting 2 in Polystigma as well as in Xylaria 
and other members of this group (e.g. Figs. 16, 20, 21). 
The width of this hypha in Poronia punctata is 4-4 /x. Further 
sections showed portions of this hypha passing up from below 
through the vegetative parts of the stroma into the coil, and 
in very occasional instances this was seen after it had formed 
one or two turns in the coil, but before it had become invested 
by the surrounding knot of hyphae (cf. Figs. 27, 19, a and b). 
After the formation of the coiled row of cells this hyphae 
grows on beyond the coil towards the surface of the stroma 
(Figs. 20, 21). This portion, however, is always more slender 
than the cells forming the coil, and though in several instances 
it was seen running right up to the surface, reminding one 
of the trichogyne of Collema, Physcia , or Polystigma , no 
evidence could be obtained that it functions as such. 
Although hundreds of serial preparations were examined, 
in no case was a section found which showed the hypha 
through the whole of its course, indeed it was rare to find 
the portions growing towards the knots shown in the same 
stroma, whose sections showed several instances of the £ tricho¬ 
gyne ’ process. This would suggest that the lower portion 
quickly disappears, or is emptied of its contents after the 
early stages of the perithecium are completed. In addition 
to the deeply stained knots of hyphae enclosing the Woronin’s 
1 Marshall Ward, Onygena equina, Willd., a Horn-destroying Fungus. Phil. 
Trans., vol. cxci, 1899. 
2 Fuisting, Zur Entwickelungsgeschichte der Pyrenomyceten. Bot. Ztg., 1867— 
1868. 
