and Fertilization in the Hemlock Spruce. 605 
branes have been deposited about the daughter-nuclei, while the last traces of the 
membrane of the mother-nucleus have disappeared. The large dense mass of 
cytoplasm at the lower pole, however, still remains, and is present even when the 
egg-nucleus begins to pass down. The formation of the cell-plate is proceeding 
pari passu with the increase in diameter of the daughter-nuclei. 
Fig. 31. (x 400.) The daughter-nuclei have reached the resting stage, and 
show a delicate reticulum with several nucleoli. The egg-nucleus is somewhat 
larger than that of the ventral canal-cell. The cell-plate has been continued to 
the wall of the mother-cell. 
Fig* 3 2 * ( x 400.) The egg-nucleus is now not only larger than the ventral 
canal-cell nucleus, but its reticulum is growing more rapidly. The chromatic 
contents of the nucleus are mostly confined to its upper portion, the lower part 
containing chiefly nuclear sap. 
Fig* 33* (x 400.) The reticulum of the egg-nucleus has become coarser 
and the nucleoli larger. The nucleus has also changed its form from spherical to 
ellipsoidal, and has begun to move through the dense polar cytoplasm toward 
the centre of the egg. The larger granules of the general cytoplasm are 
arranged in radial rows about the nucleus, and the granules themselves are radially 
elongated. 
Fig* 34* ( x 250.) At this stage the reticulum of the egg nucleus is distributed 
throughout the entire nuclear cavity. As the archegonium is unusually broad, the 
nucleus has retained its spherical form. Passing it on the left is the large central 
vacuole which now takes a position near the egg apex. The nucleus of the ventral 
canal cell is irregular in outline, and its contents show signs of disorganization. 
Traces of the division-spindle still remain above the nucleus. 
PLATE XXXII. 
Fig* 35. ( x 300.) The nucleus here represented is drawn from an egg into 
which the contents of the pollen-lube have just been discharged. Situated near 
the base of the nucleus, is the very large nucleolus with its firm, deeply staining 
outer shell broken at one point. The contents of the nucleolus appear finely 
granular and vacuolate in stained preparations. 
Fig. 36. (x 200.) The contents of the pollen-tube have entered the egg 
near its apex on the right hand side in the figure, and now lie beneath the empty 
vacuole with the functional sperm-nucleus in advance, and already free from its 
cytoplasm. Near it on the left is the stalk-cell, while between it and the sperm¬ 
cell lies the vegetative nucleus. The contents of the first sperm-nucleus are very 
dense and stain deeply. The egg-nucleus is apparently unaffected by the near 
approach of the sperm. Just above the egg are the remains of the neck-cells and 
the ventral canal-cell pushed aside by the entering pollen-tube. 
Fig* 37* ( x 200.) The first sperm-nucleus has flattened itself against the 
apex of the egg-nucleus in the form of a biconvex lens. Its contents have as yet 
undergone no change. The second sperm-nucleus remains in its cytoplasm above, 
showing its reticulum and nucleoli very distinctly. The smaller nuclei are in 
another section. The first sperm-nucleus has left slight traces of its passage to the 
egg-nucleus in the intervening cytoplasm. These traces are more distinct in 
Fig* 38. 
Fig* 38. ( x 200.) The sperm-nucleus has lost its density, and is now a true 
