622 Howard.—Trichosphaeria Sacchari , Massee. 
fifteen per cent, of gelatine. In sixteen hours a delicate 
white mycelium was observed with the naked eye at the 
points of infection and, after a copious development of white 
mycelium, chlamydospores and Melanconium- spores were 
formed. Evidently the chlamydospores enable the Fungus 
to tide over an unfavourable period, and explain the remark¬ 
able vitality of the hyphae in old specimens of diseased cane 
observed by Massee. 
Up to this point cultures of Melanconium- conidia and 
chlamydospores had reproduced one or both of these spores. 
Attempts were next made to reproduce Massee’s macro- and 
microconidia from Melanconium- spores grown in cane-juice of 
varying concentration. 
The first series of cultures was made in dilute cane-juice, 
obtained by boiling slices of ripe cane in distilled water for 
fifteen minutes, filtering and sterilizing by intermittent boiling, 
using spores obtained from a pure culture. In three days 
the flasks were filled with white septate branched hyphae, 
and in five days dark spots were noted on the matted surface 
of the culture round the margin. In ten days they were 
found covering the surface, and microscopic examination 
showed them to be stromata containing Melanconium- spores. 
The mycelium was found to be very much knotted and to 
be full of oil drops. I have very frequently noted these oil 
drops in the mycelium of the Fungus in the host plant, 
especially near a stroma. No macro- or microconidia were 
found in any of this series of cultures after the most careful 
examination. The submerged mycelium, however, was in 
many cases found to have divided into free chlamydospores. 
The second series of flask-cultures was made in sterilized 
cane-juice obtained from the clarifiers of a sugar factory. 
The juice had been heated with lime and gave a slightly 
alkaline reaction with phenol-phthalein. In seventeen days 
Melanconium- stromata were abundant, but there was no trace 
of macro- or microconidia. 
Lastly a series of flask-cultures was made in a food-material 
prepared by boiling slices of very young cane-shoots in dis- 
