Howard.—On Trichosphaeria Sacchari , Mas see, 623 
tilled water. In nine days the familiar Melanconium-s pores 
were abundant on the surface of the cultures, and the sub¬ 
merged hyphae were found to have divided up into free 
chlamydospores by the disappearance of the walls of the 
empty connexions. 
Cultures were now made in sterilized cane-slabs by infection 
with spores from a pure culture. A white mycelium spread 
out from the point of infection and in six days the mycelium 
was found to have knotted up in several places forming 
stromata, which in nine days were found to contain Melan- 
conium-SQorQS. At the edges of the cane-slabs the internal 
mycelium had formed stromata underneath the rind, which 
grew out into hairs in an exactly similar manner to that 
noted in naturally infected canes. Similar cultures made on 
blocks of sterilized oak sap-wood gave similar results. In 
eleven days the Melanconium -spores were abundant on the 
infected surface. 
All attempts to produce the micro- and macroconidial 
phases of the Fungus in sterilized media had failed. 
An extended search in cane fields where ‘ Rind Fungus * 
was present and among heaps of rotten canes in estate 
yards was now made in order to obtain specimens of macro- 
and microconidia. At length a cane was found, which, on 
being split open, showed blackening and disorganization of 
the tissues. The dark colour was found to be due to a copious 
development of Fungus-spores, which from their size and 
mode of origin agreed with the micro- and macroconidia 
described by Massee. Except for the abnormal development 
of roots at the nodes the cane in question appeared to be 
healthy till split open. Afterwards similar diseased specimens 
were obtained frcm the Marienberg Plantation, Surinam, 
through the kindness of the manager, Mr. James Mavor. 
These canes also showed a copious development of roots at 
the nodes. I have also frequently noted this abnormal root- 
development in canes which have been badly attacked by the 
‘moth-borer’ (Diatrcea saccharalis , Fabr.) and by the mycelium 
of the Melanconium-ph&se of the ‘ Rind Fungus.’ Except in 
T t 
