624 Hozvard.—On Trichosphaeria Sac chari, Mas see. 
cases where it is due to rain-water lodging between the leaf 
sheaths and the stem, it may be regarded as a sure sign of 
disease. The above specimens were kept in a moist chamber 
for some time, when they developed Melanconium -spores 
which ruptured the rind. 
In view of the occasional development of micro- and macro- 
conidia in the interior of a cane, a further attempt was made 
to reproduce these phases from the Melanconium condition. 
Two dozen healthy cane pieces were selected about two feet 
in length, and the cut ends were immediately dipped into hot 
melted paraffin to prevent the entry of Fungi and Bacteria. 
The rind of the canes was carefully cleaned and rubbed over 
with an alcoholic solution of corrosive sublimate. Two series 
of experiments were made as follows: — 
1. Six of the canes were partially split with a sterilized knife 
and in three of the slits Melanconium-s pores from a pure 
culture were sown, while in the other three growing mycelium 
from a culture of Melanconium -spores in sugar-cane extract 
was introduced. The canes were then lightly bound up with 
tape and the split end was again dipped into melted paraffin. 
Six other canes were at the same time treated in an exactly 
similar manner, except that no spores or mycelium were 
introduced, to serve as a control experiment. 
2. Six canes were split as before and a cavity was made in the 
cane by cutting out a portion of the interior with a sterilized 
knife. In three of the cavities spores were sown, while in the 
other three mycelium was introduced as before. Six other 
canes were used as a control. 
In five days all the canes which had been inoculated with 
spores were found to exhibit a considerable development of 
macro- and microconidia near the point of infection. In 
ten days all the canes which had been inoculated with my¬ 
celium gave a similar result. In no case were these spores 
found in the control canes. In nearly all the canes the split 
surface was reddish in colour. In nineteen days several of 
the inoculated canes showed Melanconium -pustules on the 
exterior and blackening under the rind due to the develop- 
