628 Howard . — On Trichosphaeria Sacchari , Mas see. 
The required culture was at length obtained by beating up 
some mycelium from a flask-culture, in which micro- and 
macroconidia were developed in about equal numbers, with 
sterile water, pouring off the upper portion and using some 
of the remainder to infect a gelatine-tube. The macro¬ 
conidia being larger and heavier, subsided more quickly than 
the lighter microconidia, and therefore the lower layers of 
water were richer in the former bodies. 
The spore germinated in five hours after sowing, and in 
eleven hours the hypha commenced to branch. In eighteen 
hours the drop was filled with a branched septate colour¬ 
less mycelium which exhibited very rapid growth. In twenty- 
four hours some of the hyphae commenced to grow down 
into the air, especially round the margin of the drop. This 
behaviour soon became general all over the drop. The 
aerial mycelium appeared olive in colour and grew with great 
rapidity. One of these aerial hyphae was fixed and stages 
in its development were obtained under the high power. 
Three hours after leaving the drop the hypha commenced 
to bend and the contents showed segmentation. Soon after 
this appearance the hypha became top-heavy and fell back 
on to the surface of the drop. This was the signal for the 
rapid liberation of a chain of greyish rectangular conidia, 
averaging 7x10//, from the distal end of the aerial hypha. 
This behaviour of the aerial hyphae was found to be general, 
and except round the edges, the surface of the drop was 
speedily covered with ejected spores. The aerial hyphae 
proved to be microconidiophores and the spores microconidia. 
When first extruded from the hyphae the conidia are greyish 
in colour, rectangular in shape and filled with granular proto¬ 
plasm. In an hour after extrusion from the conidiophore 
the protoplasm of the conidium became vacuolated and the 
conidia became rounder and larger. After this they gradually 
turned brown and in twenty-four hours became reddish-brown 
with a darker coloured central portion, and measured 15 to 
10/xx 9 to 7//. The conidiophores measured 300 /x or more 
in length. 
