Howard. — On Trichosphaeria Sacckari, Massee . 631 
The darkening of the conidia is therefore due to a change 
in colour in the spore-contents and not in the spore-wall. 
Possibly the change in colour noted in the macroconidia 
is due to the same cause. The hypha now proceeded to 
divide up by transverse walls commencing at the distal end, 
and the segments which were from 10 to 15 /x in length and 
2 to 3 /x in breadth now proceeded to round themselves off 
into a chain of spores. No further changes resulted. 
In many of the drops the old brown mycelium was seen 
to enter on a second phase of growth by sending out very 
narrow, colourless unseptate hyphae which rarely branched. 
After a time the growing end passed down into the air as 
a conidiophore which formed microspores in the ordinary way. 
Ascigerous stage. 
Extended search on diseased canes and in old cultures 
of macroconidia has up to the present not been rewarded 
by the discovery of perithecia resembling those described by 
Massee. I am preparing a series of cultures of macroconidia 
in large flasks with the hope of obtaining perithecia and 
following out the development of the ascospores. 
