792 Schwartz .— The Life-history and Cytology of 
taneously by Blomfield and the present writer ( 2 ) early in 1910. These 
accounts in their main features agree closely with the life-history of Plas- 
modiophora Brassicae as described by Nawaschin ( 4 ). In July, 1910, I pub¬ 
lished an account of the life-history and cytology of a new species, 5 . Junci , 
parasitic on the roots of the various species of Juncns , which showed a close 
agreement with that of its nearly related 5 . Veronicae . 
In January, 1911, in the ‘Annals of Botany Osborn, in a short note on 
Spongospora Solani , describes the fusion in pairs of the nuclei of the plas- 
modia prior to the nuclear divisions preceding the formation of the spores ; 
in the same number another short account of the same species is given by 
Horne. 
It is interesting to add that Plasmodiophora Brassicae has been found 
by Marchand ( 3 ) as a parasite in the roots of the Melon, Celery, and Sorrel, 
in which it caused malformations similar to those on the Cabbage. 
The material used in the course of this investigation was collected by 
myself in the neighbourhood of Sevenoaks ; some of it was fixed immediately 
after uprooting, the fixative used being usually Bouin’s picro-formol solution 
and occasionally Flemming’s solution. With a view to increasing the 
number of nuclear divisions, a number of the uprooted plants were kept 
from one to one and a half hours with their roots immersed in warm water 
at about 22 0 C., a temperature considerably above that in the open, which 
was about io° C.; on their removal from the water they were at once 
placed in Bouin’s solution. In this latter material the nuclear divisions 
were somewhat more common, although they were still few in number, and 
it needed considerable search to find any. Benda’s iron haematoxylin was 
almost entirely used as a nuclear stain. 
Occurrence of Sorosphaera Graminis. As previously stated, the first 
diseased plants I found were growing in the vicinity of Sevenoaks Station ; 
from this spot, however, I obtained only a few plants. Later on in the 
summer I was fortunate in obtaining a good supply from a locality close at 
hand, the diseased grasses being found growing as weeds along the border 
of a gravelled path in the garden of my home. The spot was shady and 
the plants were growing directly in the gravel (Kentish ragstone) of the 
path; they were mostly gathered in the late summer when the ground was 
damp. Where soil had infiltrated into the gravel the grass plants were 
free from disease and grew somewhat more luxuriantly. The situation was 
on the slope of a hill with a northern aspect, and in summer it was a dry 
one ; it was distant about a mile from the spot where I had found the 
diseased Junci . Several other likely places in the district were searched for 
diseased plants, but with no success, except in the case of a shady roadside 
close to the garden path above mentioned, and evidently forming part of 
the same diseased area, so that, as far as my experience warrants the 
expression of an opinion, the disease is not a common one. The grasses 
