Embryo and Aleurone Layer of Hordeum. 
829 
TABLE XIII. 
Relative Amylase Augmentative Capacities of Endosperms 
and Inner Endosperms. 
Total augmentation 
Relative augmentative capacities of: — 
Exp. 
of amylase. Aleurone layer. 
(equivalent to mg. of Cu .) 
Inner endosperms. 
1. 
Endosperms 
2483 
1423 
1060 
2. 
J) 
2802 
1742 
1060 
3 - 
99 
3082 
2022 
1060 
4 - 
Inner endosperms 
1060 
—• 
1060 
5 - 
» »» 
791 
— 
791 
The tentative values given in the foregoing table show that the con¬ 
tribution of amylolytic enzyme derived from the secretory activity of the 
aleurone layer forms no mean proportion of the total augmentation of 
amylase found in the isolated endosperm under the conditions of experiment 
described. 
In order further to investigate the augmentative capacity possessed by 
the inner endosperm, and for the purpose of comparison with similar objects 
under other experimental conditions, a further series of experiments was 
instituted with inner endosperms. 
In this series of experiments the copper-reducing powers of the control 
digestions of the culture medium and object were specially noted, and care¬ 
ful macroscopical and microscopical examination was made of the condition 
of the starch contents taken from various parts of the objects under culture 
at the termination of their sojourn on the plates. The object was to 
ascertain whether any marked evidence suggestive of enzymatic action, the 
presence of reducing sugars, evidence of the corrosion or dissolution of 
starch grains, or the friability of the objects was demonstrable in the inner 
endosperm contents. 
In the following table are grouped the results of the separate digestions 
of the culture medium and objects with soluble starch :— 
TABLE XIV. 
Cultures of Inner Endosperms on 5 % Gelatine-Mineral 
Salts. 
Chilian barley. Culture period, 13 days. Seeds absolute alcohol 48 hours, water 48 hours. 
Exp. 
Objects on plate. 
Amylase per 20 objects 
{equivalent to mg. of Cu ):— 
Medium. Objects. Total. 
Augmentation 
of amylase. 
1. 
5 inner endosperms (halved) 
1587 
634 
2221 
I37r 
2. 
>> n 
1031 
396 
x 4 2 7 
583 
3- 
>> >> » 
1507 
634 
2141 
I2 97 
4 - 
» » » 
2304 
238 
2542 
1698 
Amylase present initially in 
20 inner endosperms . 
844 
3 1 
