830 Stoward.—Amyloclastic Secretory Capacities of the 
In the objects themselves there were no reducing sugars in any of the 
experiments, showing that although augmentation of amylase had taken 
place it was unaccompanied by any demonstrable evidence of hydrolysis of 
the starch reserves. 
Reducing sugars equivalent to 35 mg. maltose were found in Exp. 4only, 
representing at best a very feeble amount of amylolytic action. 
Macroscopically the objects were devoid of any outward indications of 
change; they had retained their intact form and mass, and microscopical 
examination of specimens of starch taken from different parts of an object 
failed to reveal any indication of action on the starch grains. 
Thin sections of objects taken in various planes and freed from their 
starch contents by digestion at 30° C. with filtered, diluted saliva, and then 
examined microscopically, showed clearly that their cell-walls and cyto¬ 
plasmic contents were quite intact. 
After desiccation at 30° C. for twenty-four hours the objects were sub¬ 
jected to physical examination, with the result that friability of the inner 
endospermic substance, one of the usual and invariable accompaniments of 
recognizable change in the cell-walls of the amyliferous cells, was found 
to be entirely absent. 
The results so far adduced, while they show that the inner endosperm is 
capable of generating and accumulating amylolytic enzyme in its tissue, 
negative the view that this enzyme very readily or vigorously acts upon the 
semi-solid barley starch at its disposal. 
In the following table the data derived from similarly established com¬ 
parative experiments with inner endosperms and endosperms are summarized, 
the only difference in the experimental conditions between these and the 
preceding experiments being the doubling of the concentration (indicated 
by the symbol P 2 ) of KH 2 Po 4 . 
The objects were not macroscopically and microscopically examined, 
but for present purposes it is only necessary to direct attention to the quan¬ 
tities of reducing sugars in the culture media in the endosperm experiments 
of this series, since this criterion affords a far more accurate and critical means 
of forming an opinion of the relative amylolytic activities possessed by the 
amylases (whatever may be their other specific attributes) which originate 
in the inner endosperm and aleurone layer respectively, than do measure¬ 
ments of the capacities possessed by these tissues to increase their amylo¬ 
clastic enzyme content. 
