Embryo and A leurone Layer of Hordeum . 
831 
TABLE XV. 
Cultures of Endosperms and Inner Endosperms on 5 % 
Gelatine-Mineral Salts (P 2 )} 
Chilian barley. Culture period, 7 days. Seeds 72 hours absolute alcohol, 48 hours water. 
Amylase per 20 objects 
Reducing sugars 
Exp. 
Objects. (equivalent to mg. of Cu ) in :— 
per 20 in 
Medium. Objects. Total. 
medium. 
1. 
5 inner endosperms mi 317 1428 
0-00 
2. 
5 endosperms 2222 974 3196 
159 
3 . 
276 1 1349 4111 
238 
The amounts of reducing sugars found in the culture media in the case 
of the endosperm experiments, 2 and 3, afford convincing proof that when 
the aleurone layer is left intact, not only is there proof of augmentation 
of amylolytic enzyme by the endosperms, but there is also evidence of 
marked amylolytic action. 
In Experiment 1, just as in the preceding experiments with similarly 
prepared objects, there is undoubtedly an increase of amylase, but there is 
an entire absence of reducing sugars in demonstrable amount in the culture 
medium , over and beyond that initially present in the objects. 
The suggestion may be advanced that in the experiment under con¬ 
sideration the period of time to which the experiment was restricted may 
have been insufficient for the normal display of the presumed amylolytic 
function possessed by the inner endosperm. 
The experimental data summarized in the following table fail to lend 
support to such a suggestion, as shown by the results of Experiment 3, with 
inner endosperms, given in the table below. 
TABLE XVI. 
Cultures of Endosperms and Inner Endosperms on 5 .% 
Gelatine-Mineral Salts (P 2 ). 
Chilian barley. Culture periods, 3 and 17 days. Seeds 48 hours absolute alcohol, 48 hours 
water. 
Duration 
Exp. 
Objects. 
of 
culture. 
1. 
5 endosperms (halved) 
3 days 
2. 
f) t> 
17 „ 
3 - 
5 inner endosperms (halved) 
17 >> 
Amylase per Reducing sugars 
20 objects in :— in medium 
Medium. 
Objects. 
Total. 
(equivalent to 
mg. of Cu). 
1070 
1130 
2200 
0-00 
2202 
634 
2836 
313 
1309 
3 i 7 
1626 
0-00 
It may be noticed in passing that as a general result of increased 
amylolytic activity during prolongation of the culture period , in these culture 
experiments a diminution of enzyme occurs. That is, enzyme activity in 
1 KH 2 PO t concentration doubled. 
3 1 2 
