836 Stoiuard.—Amyloclastic Secretory Capacities of the 
Method (3) eliminates the possibility of the embryo secretion diffusing 
into the endosperm. 
TABLE XIX. 
Cultures of Inner Endosperms on o-8 % Agar-Mineral Salts 
(P 2 and P,). 1 2 3 
Chilian barley. Culture 
Concentration 
Duration 
Exp. 
r\i ■ a °f KH^PO, in 
Objects. J ?. 4 
J culture 
of culture 
experi¬ 
medium. 
ment. 
1. 
5 inner endosperms P 2 
14 days 
2. 
5 j »> L 
14 „ 
3 - 
>> >> L2 
20 „ 
4- 
t) )> L 
20 ,, 
5 - 
>? L 
20 ,, 
6. 
>> y> L 
20 „ 
Average initial weight of 
periods, 14 and 20 days. 
Amylase per 20 
Reducing 2 
Weight per 5 
objects per hour 
sugars per 20 
objects at 
in: — 
objects in :— 
termination 
Medium. Objects. 
Medium. Objects. 
of culture 
(equivalent to mg. of Cu.) 
experiment . 3 
0.00 379 4 
124 — 
— 
0.00 221 4 
103 — 
— 
— ' _ 
26 0-00 
167 mg. 
— — 
26 0.00 
142 „ 
85 2532 5 
113 — 
139 » 
o-oo 3798 5 
29 — 
125 „ 
inner endosperms, 175. 
Possible objections to these methods of preparation will be discussed 
under the next subsection (IV B). It may. however, be noted here that 
very little, if any, diffusion of enzyme appears to take place when intact 
seeds are steeped as previously described. 
The general character of these results, minimal depletive action, 
absence of obvious evidence of action on the cell-walls and contents, so 
closely parallels in every respect those yielded by similar objects prepared 
from intact steeped seeds that further reference to them is unnecessary. 
In Experiments 3 and 4 the objects were directly investigated for 
reducing sugars, to test whether, in the absence of demonstrable cytohydro- 
lysis of the cell-walls and the consequent presumed hindrance to the ready 
outward diffusion, the products of amylohydrolytic action had accumulated 
in the objects themselves. The foregoing experimental evidence, however, 
negatives this presupposition, and it is evident that the inferior auto¬ 
depletive capacity of the isolated inner endosperm cannot be attributed to 
this factor. 
To confirm the statement frequently employed in previous sections, that 
1 The 08 % agar solution per se was invariably faint alkaline to alizarin. The phosphate 
employed in the concentration P 2 was apparently just sufficient to render the o»8 % agar-M.S. 
neutral. 
2 The value given for the reducing sugars here and elsewhere is corrected for the small amount 
of reducing sugars present initially. 
3 The reactions of the culture medium towards alizarin at the termination of the culture experi¬ 
ment were—Experiments 1, 3, and 5, neutral, and Experiments 2, 4, and 6, acid. 
4 Determined by direct-digestion method. 
5 Determined by papain-digestion method. 
