1082 
Irving .— The Effect of Chloroform 
It will be noticed that the slope of this curve cannot be directly com¬ 
pared with those of the other experiments, because the abscissae are twice 
as extended in this figure. 
Experiment VI. Chloroform dose, loc.c. This dose of chloroform is 
very much greater than that used in the preceding experiments. The 
result is a new type of curve, without any detectable initial outburst of 
C 0 2 occurring. There may no doubt be a very brief outburst of C 0 2 , 
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Fig. 6 . 
lasting such a short time that it is masked by the fall during the rest of 
the two-hour reading. A series of one-hour estimations did not, however, 
give any evidence of an outburst of C 0 2 having occurred. 
The C 0 2 -production continued to fall off rapidly, and fourteen hours 
after chloroform had become nil. This is the extreme type of curve, E, 
characteristic of the largest doses of chloroform. 
We may now pass to a general comparison of these different types of 
chloroform-effect provided by Exps. I-VI. 
The first point for comment is the great difference in the values given 
for the normal respiration of 15 grms. of Barley leaves at 25° C., even 
though the shoots were of the same height and age in each experiment. 
After these differences were observed the matter was investigated, and was 
found to be due to the different depths of planting the seeds on different 
occasions, causing greater or less amounts of stem to be above the ground 
