io88 
Irving .— The Effect of Chloroform 
it fell steadily, but gradually, giving a curve of a type D-E. This is seen 
in Fig. 13. The hatching at the bottom of the figure shows that the 12 c.c. 
of chloroform put in the bulb-tube was all vaporized in' eight hours. 
Experiment XII. Concentration of Chloroform — 4 c.c . per hour per 
litre of air-current. As 1*5 c.c. did not give the extreme form of curve, 
type E, still stronger concentration was obtained. For this the bulb-tube 
was inadequate and the procedure of Section I was ^employed, 4 c.c. of 
chloroform being introduced every hour into the dish at the bottom of 
the glass jar. This amount was repeated for fourj consecutive hours, 
00*2 
0040 
0038 
0036 
0034 
0032 
(/} 0030 
2 0028 
a 0026 
(J) O 024 
2 0022 
" -0020 
g 0018 
•0016 
0 -0014 
I- 0012 
gj OOIO 
I- 0008 
q 0006 
0004 
•0002 
•OOOO 
Hours 0 2 4- 6 8 IO 12 14 16 18 20 22 24 
Fig. 13. 
Fig. 14. 
i.e. four lots of 4 c.c. of chloroform were run into the leaf-chamber: sub¬ 
sequent to that no more chloroform was given. 
With this strong dose, the Barley showed no sign of an initial outburst 
of C 0 2 , but fell off steadily towards zero, reaching it about sixteen hours 
after the beginning of chloroforming. 
The type of curve is the same as that found for Exp. V and called 
type E. The results of this experiment are plotted in Fig. 14. This 
experiment completes our series and we have passed from A to E by 
different concentrations of chloroform. 
We may now refer to two experiments, XIII and XIV, performed 
to bring out certain special points. 
When the outburst of C 0 2 caused by chloroform is very transitory 
and followed by a rapid fall, then its occurrence will not be easy to 
