1093 
upon Respiration and Assimilation . 
To see how much assimilation was now actually taking place, the leaf 
was darkened, when the output rose at once to 0-0013 grm., showing that 
the leaf had been assimilating some two-thirds of its respiratory C 0 2 . This 
trace of chloroform therefore had at once very much reduced the function 
of assimilation, after which the leaf partially recovered. The amount of 
chloroform was not enough to completely abolish this function, as the C 0 2 - 
output in the light never rose to be as great as the respiratory output in the 
dark. It is very striking that so small a dose, which would presumably 
have no effect upon respiration, is sufficient to reduce assimilation nearly 
to nothing, and that too when the dosing only lasts four hours. The leaf 
remained bright green throughout the experiment. 
Fig. 17. 
Fig. 18. The hatching which indicates chloroform has been accidentally omitted at the bottom 
of this figure : it should extend from the fourth hour to the end of the experiment. 
Experiment XVL Chloroform concentration — 0-02 c.c. per litre of 
air-current. In this experiment it was decided to chloroform the leaf in 
the dark first, and then to illuminate it and see if there would be a fall 
in the C 0 2 -output as evidence of some residual power of assimilation. 
