1097 
tip on Respiration and Assimilation. 
that after a sufficient moderate dose of chloroform, the curves of output 
of C 0 2 became practically identical, whether the leaf was illuminated in 
a light strong enough for assimilation in a normal leaf, or was completely 
darkened. The leaf in the light weighed 2*95 grms., that in the dark 
2-8 o grms. 
Both leaves turned brown and had drops of moisture on their under¬ 
surface at the conclusion of the experiment. 
Experiment XXL Chloroform concentration , about 0-25 c.c. per litre of 
air-current for one hotir. To continue the series by giving still stronger 
doses, 0-5 c.c. of chloroform was introduced straight into the anchor tube of 
the chloroform apparatus, the middle arm being then corked. This amount 
was completely vaporized in less than one hour. 
Fig. 23. 
As this experiment was a double one (Fig. 23), with one leaf in the 
light (weight 2-1 grms.) and the other in the dark (weight 2-15 grms.), each leaf 
was subjected, for about an hour, to a concentration of chloroform of about 
0-25 c.c. per litre of air. The usual outburst of C 0 2 was produced in both 
leaves. Although the chloroform supply then ceased, the output of C 0 2 
fell rapidly to zero, as it would have done had the chloroform been kept on 
continuously. The leaves turned a chocolate colour, and exuded a quantity 
of water, so that they were very flaccid at the end of the experiment. 
Experiment XXII. Chloroform concentration , about 0-75 c.c. per litre 
of air-current for one hour. The procedure for this double experiment 
was the same as in Exp. XXI, but 1-5 c.c. of chloroform was introduced 
into the anchor tube and vaporized in an hour. The leaf in the light 
weighed 2-5 grms., that in the dark 2*31 grms. 
