Embryo and A leurone Layer of Hordeum . 1179 
does not exercise any influence on the amylase present in the resting grain 
(compare Experiments 1, 4, and 5)- 
At first it appeared to be probable that the inactivation produced 
by boiled barley substance in the above and preceding experiments might 
be due to adsorption of enzyme. This suggestion, however, is dispelled by 
the results of Experiments 4 and 5, and. moreover, Experiment 3, in which 
double the quantity of boiled barley substance was used, fails to lend 
support to such a surmise. The reaction of the boiled barley substance was 
approximately neutral to such indicators as alizarin and methyl-orange ; it 
is therefore difficult to ascribe the results observed to change in the reaction 
of the digestion medium. 
The interpretation of these results is a matter of difficulty ; their general 
trend suggests the possible presence of an anti-enzyme, an anti-amylase, 
or of a phytotoxin, either of which might possibly be unmasked by boiling 
the finely-ground barley substance. 
Further experiments were then instituted, the barley substance being 
subjected to one or other of the treatments set forth in the following table :— 
P . Amylase equivalent 
p ' to mg. Cu. 
1. o-2 grm. barley + 25 c.c. 6 % starch solution + 5 c.c. water 316 
2. 0-2 „ 
„ + 25 C.C. . 
.1 ■ » +5 c.c. 
o«2 grm. air-dried barley 
heated 5 hours at 1 oo° C. 
in sealed tube 
321 
3 ' 0*2 » 
„ + 25 C.C. 
„ ., +5 c.c. 
,, o-2 grm. barley heated in 
sealed tube, 5 hours at 
100° C. 
190 
4. 0.2 „ 
„ +25 C.C. 
„ „ +5 c.c. 
,, 0*2 grm. barley heated 
under mercury seal 5 
hours at ioo° C. 
200 
5. 0.2 „ 
,, +25 C.C. ! 
■> » +5 c.c. 
„ 0.2 grm. barley heated 
5 hours at ioo° C. under 
open-air condenser 
205 
These results do not differ materially from those given in the preceding 
table, with the exception of Experiments 3, 4, and 5, which show that more 
prolonged heating of barley substance in presence of water tends to reduce 
its retarding effect. As before, when barley substance is heated in the air- 
dried condition (Experiment 2) the retarding influence which it previously 
exercised is entirely annulled. 
Experiment 4 of the preceding and Experiment 2 of the above series, 
in which the air-dried substance was freely exposed to air and sealed 
respectively, seem to show that the inhibitory agent is not a volatile body. 
The following series of experiments were essayed with the object of 
ascertaining whether the amylase which accumulates on predigestion with 
papain and is presumed to be due chiefly to the transformation of ‘ latent 5 
into ‘ free * enzyme would in any way modify the character of the results 
previously obtained. 
