H93 
Embryo and Aleurone Layer of Hordeum . 
subsequent determinations of the copper-reducing values, and the method 
of expressing the results, are precisely identical with those already cited. 
In the following table are shown the results derived from the estimation 
of intact seeds after three and a half to six days’ germination :—- 
TABLE XLV. 
Amylase Content of Intact Germinated Seeds. 
Digestion method. 
Papain 
Auto 
Direct 
Germination period. 
?>h days- 6 days. 
Amylase per 20 objects per hour 
(equivalent to mg. Cu ). 
7682 9001 
5664 10161 
5044 8148 
There is, as the above values show, a decided tendency as germination 
progresses for the values of the amylase content of the seed taken as a whole 
to converge ; at the end of three and a half days the divergence as exhibited 
by material from air-dried or steeped seeds is very considerably diminished ; 
at the end of six days the values are of practically the same order of magni¬ 
tude, such divergence as is still existent being almost certainly due to the 
individuality of the seeds taken for examination. 
The results comprised in the table which follows throw some light 
on the relative distribution of amylase in the structural parts of the seed, 
and moreover indicate where the divergence mainly exists, namely, in the 
inner endosperm tissue. 
The interesting fact, exemplified not only here but in the table which 
follows, is brought to light, i. e. that the amyloclastic capacity of the 
embryo is greatest as measured by the direct method of digestion; the values 
afforded by the aleurone layer tend to approximate, and those for the whole 
seed, whether determined by direct experiment or calculation, also show 
a similar tendency. 
TABLE XLVI. 
Amylase Content of (a) Structural Parts from Germinated 
Seeds, (b) Intact Germinated Seeds. 
Digestion 
method. 
Germination period, 4 days. 
r. , Aleurone Inner 
m ryos. i a y ers% endosperms. 
Amylase per 20 objects per hour 
(equivalent to mg. Cu). 
Total. 
Papain 
232 
2405 
8924 
11561 
Auto 
3 xo 
2560 
5742 
8612 
Direct 
465 
x 55 2 
4 I 9 ° 
6207 
Intact seeds. 
Amylase per 20 objects per hour 
(equivalent to mg. Cu). 
10550 
8846 
675 * 
A repetition of these characteristic features is shown by the experimental 
data embodied in the table which follows:— 
