Nichols—Binuclcated Cells'in Some Basidiomycei.es. 35 
whole with about a quarter of an inch of the substratum. 
Larger myeelia were cut into small pieces for fixation. In order 
to force the fluid through the thick felt which the mycelium 
forms the material was placed in a small bottle and well cov¬ 
ered with the fixing fluid. The bottle was then fitted into the 
end of a rubber tube which was connected with the air pump. 
The air was pumped out from the closely matted hyphae, after 
which the fixing fluid was renewed. 
The material was fixed in Flemming’s solutions both the 
stronger and the weaker. Merkel’s and Herman’s solutions 
were also used. The best results were obtained from Flemming’s 
weaker solution. Both Flemming’s triple stain and Heiden- 
hain’s iron haematoxylin gave satisfactory results. 
Ilypholoma 'perplexum, Pk. 
The spores of Ilypholoma perplexum were collected in great 
abundance. The carpophores appeared in great profusion on 
decaying stumps and logs of oak throughout September and Oc¬ 
tober. The spores were collected and stored after the manner 
already described. A large per cent of the spores obtained from 
mature pilei germinated in a shorter time than those obtained 
from younger ones. The spores were sown in the hanging drop 
agar cultures made with bean decoction. The cultures w r ere kept 
in a dark box at a temperature of 20° c. As all the spores do 
not germinate at the same time but vary from four to forty-eight 
hours in the time of the appearance of the germ tube a large 
number of stages are obtained from the same slide. 
The mature spores were studied in the drop cultures and also 
in the cross sections of the gills where they were still in connec¬ 
tion with the sterigmata. 
The mature spore of ilypholoma perplexum has a dark brown 
opaque w T all which before germinating swells to two or three 
times its original thickness becoming much lighter in color and 
transparent. At this time it is easy to distinguish two nuclei 
lying close together near the center of the spore. They show 
the Usual structure of the resting nucleus and are small spherical 
bodies with a w 7 ell defined membrane, a large distinct nucleole 
