36 Wisconsin Academy of Sciences, Arts, and Letters. 
and a fine granular chromatin which fills the remaining space 
(Fig. 1). The chromatin stains a light blue with the triple 
stain while the nucleolus stains with safranin. Previous to 
germination there is no indication of a germ pore but the germ 
tube always appears at the same place on the spore. On germ¬ 
inating a tube in the form of a large round bulb of protoplasm 
is protruded from the end opposite the point of original attach¬ 
ment to the sterigma. The germ tube increases rapidly in size 
retaining its spherical form as the cytoplasm passes into it from 
the spore. One of the nuclei passes from the spore into the 
tube as soon as there is room (Fig. 2 & 4) the other may pass 
out at once and it will then be seen near the first (Fig. 3) or it 
may remain in the spore for some time. Strands of cytoplasm 
caused by streaming from the spore into the tube are clearly 
shown in many of the preparations. As the nucleus which has 
remained in the spore is carried along by the streams of cyto¬ 
plasm it becomes drawn out into a blunt point on the side to¬ 
wards the germ tube. (Fig. 2). As the nucleus approaches the 
narrow passage from the spore into the germ tube it is very 
nearly cone shaped. The vacuoles are very small at this stage 
with the exception of a single large vacuole which forms in 
the spore back of the second nucleus. 
The tube begins to lengthen immediately in the direction of 
the long axis of the spore. The bulb has not formed a firm wall 
at this time and as the tube lengthens it becomes drawn out 
in the general direction of growth until its diameter is only 
slightly greater than that of the spore. The two nuclei do not 
remain together, one moving towards the apex of the tube -while 
the other remains near the base. One of the preparations at this 
stage showed late division figures (Fig. 5). The chromosomes 
have already passed to the poles so that it is impossible to de¬ 
termine their number. The spindle fibres still connect the two 
masses. The two nuclei lie at some distance from each other. 
One spindle which is near the tip is nearly parallel with the 
walls of the germ tube. The spindle of the other, near the spore, 
is placed obliquely. Near one of the nuclei are two small deep 
staining bodies which may be remnants of the old nucleoles. 
(Fig. 5a). In an older germ tube the next division was ob- 
