Nichols—Binuclcalcd Cells in Some Basidiomyceles. 37 
served. In this hypha three nuclei were in the resting condi¬ 
tion. One is somewhat separated from the others towards the 
tip of the hypha and is a densely staining homogeneous body. 
Two other nuclei side by side near the center are elongated with 
the nucleole at one end. The fourth nucleus is dividing and 
is in the equatorial plate stage. The separate chromosomes 
could not be distinguished. The spindle fibres show an even 
distribution at the center and are collected into a definite pole. 
The presence of a central body could not be distinguished 
definitely (Fig. 6). 
As the germ tube elongates the cytoplasm forms a much thin¬ 
ner layer at the periphery of the cell and a number of large 
vacuoles appear. The number and size of the vacuoles increase 
until they are only separated by fine lamellae of granular cyto¬ 
plasm. The nuclei multiply rapidly and are frequently in pairs 
for some distance but this arrangement is not at all uniform. 
The nuclei may be separated from each other by long distances 
or they may lie in groups of three or four. Cross partitions 
are not formed in the young germ tube. It frequently branches 
two or three times before the first cross wall is formed. The 
cells which are then formed are very variable in length. The 
number of nuclei which they contain is from one to four (Fig. 
7). The mycelium does not consist in these earlier stages of 
regularly uninucleated or binucleated cells. The mycelium 
grows rapidly and branches freely and the branches interlace 
forming a loose net-work. The branches do not show any reg¬ 
ularity in their origin. A few are formed near a cross wall but 
the majority bud out somewhere near the center of the cell. 
The majority do not show a cross partition at their base. The 
first cross wall appears at varying distances up the branch. 
Usually a nucleus is found at the base of each newly forming 
branch but this rule is not constant for in a few cases a fairly 
long branch was found without any nucleus near. The lateral 
branches are narrower than the hyphae from which they arise. 
The first few cells of the branch contain as a rule fewer nuclei 
than the cells in the main hypha but the cells nearer the tip 
may have a large number. The number of nuclei at this stage 
varies from one to eight or nine; the same branch frequently 
