42 Wisconsin Academy of Sciences, Arts, and Letters. 
When the basidia are formed there is an uninterrupted series 
of binueleated cells from the binueleated cells of the selerotium 
through the stipe, pileus, trama and subhymenium to the basidia. 
Later the cells of the stipe and pileus contain from six to eight 
nuclei. 
The stages of nuclear fusion and subsequent division in the 
basidium have been fully described by Wager, ITarper and 
others. In nearly all the essential points Hypholoma perplexum 
agrees with the forms studied by these authors. The nuclei are 
in the spirem stage at the time of fusion. The fusion nucleus 
moves to the summit of the basidium when it divides. The pro¬ 
cess of the formation of the spindle was not studied. The 
spindle is always at a right angle to the long axis of the basidia. 
The chromosomes in the equatorial plate show a tendency to 
aggregate into a number of larger masses probably as a result 
of fixation. In every case there are eight or more. Certainly 
there are many more than two as claimed by Maire. 
The second division follows the first very closely. The chro¬ 
mosomes in this case are not scattered on the spindle but defin¬ 
itely arranged in an equatorial plate. The four nuclei move to 
the center of the basidium. At this time or a little later fibres 
extending from, the sterigmata to the nuclei at the center were 
observed in a large number of basidia, but their origin was not 
worked out. As to these fibres Maire says ‘ ‘ Apres la formation 
des noyaux-fils definitifs la baside, ceux-si se massent le plus 
souvent a la base ou au milieu de la, cellule, tandis que les cent- 
rosomes restent au sommet si les mitoses etaient apicales, s’y 
rendent dans le cas contraire. En face de chaque centrosome 
apparait rebouche se produit une differenciation kinoplasmique 
qui s;e propage jusque’ aux novaux at meme quelquefois plus 
loin vers la base de la cellule, orientant ■ tant le cytoplasma de 
la baside par rapport aux sterigmates. ’ ’ 
Each spore receives a single nucleus which immediately divides. 
In a number of cases the spindle is at right angles to the long 
axis of the spore. The chromosomes both at the equatorial plate 
and where they are drawn back to the poles appear to be four 
or more. 
