Nichols-—Binucleated Cells in Some Basidiomyceles. 45 
edge of the agar the terminal cells become smaller and contain a 
large number of nuclei closely packed together. 
Arising from some of the cells near the base of the primary 
hyphae are short thick eonidiophores. Their cells are nearly as 
broad as they are long. The end cells bear one or two very short 
branches which produce clusters of very slender hyphae formed 
of cells two or three times as long as broad. The cells separate 
readily into rod shaped oidia (Fig. 12). These hyphae do not 
appear above the surface of the substratum. 
Near the center of the oidiurn there is a small well defined 
nucleus in which the nueleole and granular chromatin can be dis¬ 
tinguished. The remainder of the ceil is filled with finely vacu¬ 
olated cytoplasm. This is in marked contrast with the form 
found by Maire on C opr inns radiaius. lie says ‘ ‘ Chacpie oidie 
renferme un seul noyau reduit d’ordinaire a une petite masse 
homogene chromatique et un grande vacuole.” On account of 
the number of oidia I was unable to see the nuclei in the cells of 
the supporting branch. 
Occasionally the cells of the main hypha are binucleated. In 
this case the first few cells near the spore have from one to five 
nuclei. The cells further from the spore are regularly binucle¬ 
ated. At the end of five or six days branches appear on some of 
the older cells near the spore that are much larger than any pre¬ 
viously formed and are frequently club-shaped. The cells of 
these branches are binucleated, the nuclei lying near together, 
sometimes side by side, near the center of the cell. The branches 
with binucleated cells may be formed from any cell of the myce¬ 
lium near the spore. Fig. 13 shows one of the branches with 
binucleated cells formed on a hypha with uninucleated cells. 
The branch is formed very near the center of the cell instead of 
at one end as is usual with the mycelial branches. In my opin¬ 
ion the newly formed branch probably received a single nucleus 
which immediately divided. Fig. 14 is drawn from a hypha 
with multinucleated cells showing one of the larger branches just 
forming. Two nuclei of the multinucleated cell are just at the 
base of the newly forming branch. 
Fig. 15 shows a group of these branches closely crowded to¬ 
gether—the first indication of a fruit body. The hyphae have 
