Nichols—Bmucleatcd Cells in Some Basidiomycetes. 55 
Towards the surface these large hyphae become much fewer and 
are separated by other hyphae w T hich are only slightly smaller 
but very rich in protoplasmic contents. This second form of 
hyphae are the lateral branches of the first and correspond to 
the slender hyphae of the other species but are very different in 
appearance. They are loosely twined together towards the in¬ 
terior, forming an open mesh work through which a few of the 
larger hyphae run and also leaving numerous air-spaces. 
Towards the outside they become more and more closely woven 
together until they form a layer of densely packed hyphae just 
beneath the surface. Their cells are not so long as in the large 
hyphae but are nearly as wide in many places. They are irreg¬ 
ular in width, especially towards the surface and contain large 
numbers of deeply staining bodies probably of a proteid char¬ 
acter. These bodies take a deep blue color with the triple stain 
which requires a long exposure to Orange 0. to remove. On ac¬ 
count of the number of these dark bodies it was possible to locate 
the nuclei in only a few of the cells. In favorably stained cells 
there were two large nuclei with the usual structure, a small 
nucleole, and very finely granular chromatin, surrounded by a 
nuclear membrane with a deeply staining body at one side. 
The hyphae. do not have their walls thickened when they reach 
the surface as in all of the preceding species but their cells be¬ 
come shorter and very vesicular. The hyphae are very loosely 
w r oven together forming a very thick outer layer. The cells do 
not contain any of the dark bodies so abundant in the layer just 
beneath, but have a single large nucleus, which is very irregular 
in shape, near the center of the cell. This large nucleus is im¬ 
bedded in a central mass of cytoplasm from which strands radi¬ 
ate in every direction towards the walls, as is the case in 
Spirogyra cells. I did not find any crystals in any of the cells 
or any convex plates at any of the cross walls. 
Lycoperdon pyriforme. Schaeff. (Fig. 23.) 
The mycelial strands of Lycoperdon pyriforme were found 
growing in a rotten stump. By breaking off pieces of the wood 
large quantities of the strands were obtained free from foreign 
