Reed—Infection Experiments. 117 
TJ. S. Department of Agriculture. The seed of the cereals was 
obtained from Prof. R. A. Moore, Agronomist of the Wisconsin 
Agricultural experiment station. 
The seed obtained from these various sources, in some cases, 
was not pure. Great care had to be taken to have only seedlings 
of the desired species of grass for the experiments. The seed¬ 
lings of most of the grasses used have certain distinctive charac¬ 
ters by which they can readily be distinguished. The greatest 
difficulty was with the various species of Poas as the young seed¬ 
lings are quite similar in general appearance. 
To test the results reported by Marchal spores from rye were 
sown upon wheat, oats, barley, E. jubatum, Bromus mollis, Poa 
pratensis, P. irivialis, P. nemoralis and P. compressa. The re¬ 
sults of these experiments are given in the folowing table: 
Table I .—Conidia from Secale cereale. 
Plants on which spores 
were sown. 
Total 
No. of 
experi¬ 
ments. 
Atre of 
seed¬ 
lings. 
(Days.) 
Total 
No. 
inocu¬ 
lated. 
Results. 
Rye. 
On inoc. 
seed¬ 
lings. 
On 
controls. 
No. of 
trials. 
No. 
suc¬ 
cessful. 
Triticum vulgare . 
24 
7-12 
66 
— 
— 
20 
18 
A vena sativa . 
16 
8-19 
49 
- 
- 
16 
14 
Hordeum vulgare . _ 
15 
11-16 
45 
- 
- 
11 
11 
Hordeum j ubatu m . 
8 
16-56 
32 
- 
- 
8 
8 
Bromus mollis . 
5 
28-56 
12 
- 
- 
5 
5 
Poa pratensis . 
12 
16-101 
75+ 
-? 
- 
10 
10 
Poa trivialis . 
6 
26-44 
35+ 
- 
- 
6 
6 
Poa nemoralis . 
9 
52-110 
40+ 
- 
8 
8 
Poa compressa ... 
5 
26-44 
16 
— 
— 
5 
5 
The sign — indicates no infection; —? indicates that in one experiment there was, ap¬ 
parently, infection, an account of which is given below. The bell jar was left over the 
seedlings either for 24 hours or 48 hours, or, in many cases, during the whole course of 
the experiment. Three to five different kinds of grasses were placed under the same 
bell jar, one of them always being rye; consequently in the table the record of the 
observations on the rye is repeated in several cases. The actual number of experiments 
with rye recorded in the table is fifty-two. Only two were unsuccessful. 
These were all made by sowing spores on young seedlings and 
placing them in a moist atmosphere under a bell jar, except four 
of the experiments with wheat and two of those with blue grass 
which were made by Salmon’s Petri dish method described 
