Marquette—Spore Mother-Cells of Marsilia. 
85 
drawing is made, a number of starch grains, arranged in a 
single row, extend fully halfway around the nucleus. 
The starch grains at this time stain a deep blue, and are 
not as closely packed as at a somewhat later stage. The more 
or less cylindrical region lying between nucleus and outer cell 
wall contains, in addition to the starch grains, strands with a 
granular structure which run here and there between the starch 
grains, probably forming more or less of a network. Besides 
these strands, there seems to be a diffuse, finely granular, blue- 
staining substance scattered throughout this region. It is dif¬ 
ficult to make out with certainty the structure of the material 
between the starch grains, because the latter are numerous and 
stain heavily, so that at any particular focus considerable light 
is cut off by the overlying and underlying portions; but it seems 
certain that there is a finely divided substance staining blue in 
the triple stain between the starch grains. The abrupt ending 
of this material outside of the starch grains seems to be at least 
to a considerable extent responsible for the fairly sharp delin¬ 
eation of this region against the remainder of the cytoplasm. The 
cytoplasm as a whole is still rich in its more solid constituents, 
although the proportion of vacuolar space has become somewhat 
larger than it was in the earlier stages. The strands have be¬ 
come more drawn out, look more like fibers, and many of them 
show perhaps a greater affinity for the blue stain. Also con¬ 
spicuous at this time are unusually long strands, which fre¬ 
quently lie in groups, converging at one end and spreading 
apart at the other. In addition, there are dense-staining gran¬ 
ules scattered here and there in the cytoplasm. 
The cell apparently has reached its full size, although the nu¬ 
cleus, which at this stage lies near the inner cell-wall, 
seems as yet noticeably smaller than at the time of synapsis. 
The chromatin is in the form of , strands of fairly regular out¬ 
line and uniform diameter, which loop and coil around con¬ 
siderably, often traversing the nuclear cavity. Free ends of 
such strands are not apparent, although if they lay at the 
points where the strands come in contact with the nuclear mem¬ 
brane they could easily be overlooked. 
