Marquette—Spore Mother-Cells of Marsilia. 89 
that numerous deeply-stained blue granules lie close against 
the inner side of this part of the nuclear membrane (Fig. 4). 
They are rarely if ever more than one layer deep, and, although 
small, stain intensely, so that they are readily made out in a 
tangential section of the nucleus. These granules are found 
only under the apparently thicker parts of the nuclear mem¬ 
brane, and undoubtedly are partly responsible for the heavier 
appearance of this part. As will presently be seen, the arrange¬ 
ment of the cytoplasmic fibers outside of the nucleus is also such 
as to make this part of the nuclear membrane appear 
thicker. Blue-staining fibers have become conspicuous in the 
cytoplasm and form an intertwining network of relatively long 
fibers, lying for the greater part close to the nucleus. The 
fibers have a distinctly different appearance now from that pre¬ 
sented by the fibrous constituents of the cytoplasm in the earlier 
stages of the development of the spore mother-cells. They have 
become smoother, take a brighter blue stain, and stand out in 
greater contrast to the other constituents of the cytoplasm; 
here and there they show thickenings and granules lying against 
them. In a tangential section taking in just a small piece of 
the surface of the nucleus, these fibers can be seen in great 
numbers forming a loose tangle about the nuclear membrane. 
A median section of the cell, however, taken in such a plane 
that it includes the nueleole at the focus of the chromatin 
strands, in other words in a plane including the main axis of 
the cell, shows that the cytoplasmic fibers are not uniformly 
distributed about the nucleus (Fig. 4). At the side opposite 
the starch mass they lie closely packed against the nuclear 
membrane, so closely that frequently it is difficult to make out 
the individual fibers; the appearance at times suggests that the 
cytoplasmic fibers are separating, splitting off” from this 
denser part of the nuclear membrane. As they approach the 
region of the starch mass, the fibers separate more from each 
other and extend farther from the nucleus. When they reach 
a plane drawn a little above the nueleole at the focal point of 
the chromatin strands and perpendicular to the main axis of 
the cell, the fibers frequently bend rather sharply in towards 
the edges of the starch mass, at times giving rise to a grouping 
