Andrews—Experiments with Larva of the Bee Moth. 257 
37° C. In a second const ant-temperature compartment the tem¬ 
perature of 37° was obtained by using a 40-watt and a 60-watt 
Mazda light. Newly hatched larvae were put in tall glass jars 
containing brood comb and placed in this constant temperature 
compartment on October 28, 1916. They seemed to be normal and 
throve well. Pupation took place, the moths emerged, mating 
occurred, and eggs were laid. About fifty per cent, of the eggs 
did not hatch, but seemed dried up by the heat; the rest hatched 
into normal larvae. The length of life of the moths was about four 
days for females and six days for males. The females sometimes 
emerged before the males, a condition which was never found to 
obtain among the moths kept at 29°C. From the time when the 
larvae were put in the incubator until May 29, 1917, the life cycle 
had been completed five times. At no time during the experiment 
were there less than two hundred and fifty larvae in our dishes. 
At each instance of egg-laying under this temperature, the same 
difficulty of the eggs not hatching was found. The heat did not 
seem to affect the young larvae after they had hatched. 
45°G. In the third constant-temperature compartment the de¬ 
gree of temperature desired was produced by using two Mazda 
globes of 60 watts each, and one of 40 watts. On March 10, 
1917, nearly mature larvae were put in glass jars containing 
brood comb, and the latter were placed in this compartment. A 
dish of water was also put in the incubator. At the end of forty- 
eight hours, the larvae appeared healthy and were spinning co¬ 
coons. A day later they seemed to be dying, and ninety-six hours 
after they were first put in, the larvae were all dead. About 
twenty-five were used for the experiment. It did not seem neces¬ 
sary to repeat it, because if the older larvae, which are hardier 
than moths, young larvae, or eggs, could not withstand a tempe¬ 
rature of 45°C., the other forms surely could not. 
Table 1 gives the result of the experiments. 
17—S. A. L. 
