Munro—Digestiva Secretions of Pickerel and Perch. 271 
tubes. A test with copper sulphate showed by the rose reaction 
that in the tubes containing pancreatin and gycerine extract of 
the intestine proteoses and peptoses were present, whereas in the 
other two tubes there was no evidence of their presence. 
With cane sugar. Four test tubes were set up, containing, 
respectively: (1) 2 cc. cane sugar solution and 30 drops of 
glycerine extract of the intestine; (2) 2 cc. cane sugar solution 
and 30 drops of glycerine extract of the pancreas; (3) 2 cc. cane 
sugar solution and 30 drops of commercial pancreatin solution; 
(4) 2 cc. cane sugar solution. Ten cc. of a 1% solution of sodium 
carbonate were then placed in each tube and the tubes were 
placed in a bath at a temperature of 30° C. for one hour. At the 
end of this time the contents of each tube were treated with 4 cc. 
of Fehling’s solution. The latter was soon completely reduced 
by the contents of the tube in which commercial pancreatin had 
been placed, whereas no reaction was obtained in the other three 
tubes although they were allowed to stand for several days. This 
showed that the cane sugar had been inverted to dextrose by the 
action of the commercial pancreatin solution only. 
Bile. The action of the bile in emulsifying oils was tested, and 
it was found that one drop of the bile of the pickerel was suffic¬ 
ient to form a thick emulsion with 10 drops of olive oil. 
Pergh 2 
Similar experiments to those described for the pickerel were 
performed with glycerine extracts of the enzymes of the perch. 
Since, however, no structure which can be definitely identified as 
a pancreas had been found in the perch (Pratt, 1905), it was 
possible to run these experiments with only two extracts, those of 
the stomach and the intestine. The combined secretions of two 
fishes were used on account of the small size of each fish. 
Peptic Digestion 
Three test tubes were set up, containing, respectively: (1) 23 
drops glycerine extract of stomach and 0.2 gram egg albumen; 
(2) 23 drops commercial pepsin solution and 0.2 gram egg al¬ 
bumen; (3) 0.2 gram egg albumen. All were filled with a 2% 
solution of hydrochloric acid and placed in a bath at a tempera¬ 
ture of 28° C. for 40 minutes. The contents of the tube contain- 
2 Perea flavescens Mitchill. 
