392 Wisconsin Academy of Sciences, Arts, and Letters. 
tinctly ovoid with the broader end in advance. The nucleole very 
soon takes up its position at the narrower end of the nucleus 
(figs. 24, 25). Two of the nuclei are soon observed to lag be¬ 
hind and plainly indicate by their staining reaction that they 
are degenerating (figs. 25, 26). In sections containing basidia 
which have already produced spores, it is possible to find traces 
of the degenerating nuclei, thus precluding the possibility of a 
second set of spores being produced as has been suggested by 
some observers. 
As the sterigma elongates it gradually decreases in diameter, 
and the nucleus at the same time elongates and becomes finally 
almost rod-shaped as it passes into the developing spore. There 
is at no time any indication of a centrosome or comparable body 
aiding in the formation of the young spore as described by Le¬ 
vine (26, 27), Fries (13, 14), and others for the higher Basidio- 
mycetes, but the process is very comparable to that described 
by Fitzpatrick (11, 12). As the spore grows to maturity the 
nucleus assumes a central position and increases greatly in size. 
When fully developed, the spore is seemingly separated from the 
sterigma with some force, as is shown by a spore print which 
extends to some distance beyond the boundary of the fruiting 
body. 
Summary 
1. These studies have been based upon material from three 
distinct species of Dacrymyces. 
2. The spores of Dacrymyces when thrown from the sterig- 
mata are one-celled and contain a single nucleus each. 
3. Under proper conditions the nucleus divides and septa are 
formed, producing an eight-celled spore. 
4. The spore often produces hyphae in the four-celled stage. 
5. The division of the spore nuclei is quite like nuclear division 
in other Basidiomycetes. 
6. Four chromosomes are typically found, the nucleole is ex¬ 
truded into the cytoplasm, and centrosomes are present. 
7. In cultures, binucleated cells are rarely found, anastomoses 
are present but no nuclear migration has been noted. No clamp 
connections have been observed. 
