72 Wisconsin Academy of Sciences, Arts , and Letters. 
tral bodies are conspicuous but apparently somewhat variable 
in size. Occasionally they appear saucer shaped with the con¬ 
cave side toward the spindle. It is noticeable that the spindle 
fibers seem thicker and more deeply stained toward the poles. 
The polar radiations are short and rather few in number, but 
are sharply differentiated. Among the bases of the polar rays, 
the protoplasm) is denser, forming thus an oval mass, which lies 
beyond rather than around the central body. The chromo¬ 
somes are quite irregular in shape and grouping. There ap¬ 
pears to be no stage in which they are aggregated in a flat plate. 
They seem, always more or less strung out along the length of 
the spindle (Figs. 18 and 19). Frequently two or more chro¬ 
mosomes overlap each other and lie so close together that they 
cannot be clearly differentiated. It. is hence impossible to de¬ 
termine their number with certainty. How r ever there can be 
no doubt that the number is greater than two as described by 
Poirault and Raciborski for C oleosporium Euphrasiae and the 
rusts generally. The evidence drawn from, our preparations 
indicates that their number is somewhere between six and ten. 
When the chromatin masses withdraw to the poles, which they 
do quite irregularly, the axis of the spindle lengthens some¬ 
what. At this stage the polar radiations seem somewhat longer 
than before and do not form so regular an aster. The daugh¬ 
ter nuclei are reconstructed in the usual manner and the promy- 
celial cell now divides so that the two nuclei are separated and 
we have a two celled stage as shown in Fig. 5. After the cell 
division is complete the daughter nuclei divide almost immedi¬ 
ately. The division of the two is nearly simultaneous though 
not entirely so (Fig. 23). These nuclei are very much smaller 
than the original parent nucleus. Their mitotic figures are 
also much smaller and consequently less favorable for study. 
Still it can be seen that the stages of division are essentially the 
same as in the division of the fusion nucleus. The chromosomes 
are so massed that it is impossible to distinguish their individ¬ 
ual outlines. The central bodies and polar asters are however 
very sharply differentiated. The rays of the latter are long, 
reaching in some cases almost to the periphery of the cell and 
in some cases curiously curved at their tips (Fig 23, the lower 
